Glo Substrate Reagent Pack Summary
INTENDED USE & DESCRIPTION
Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.
This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes.
Background: Buffers, Chelators and Reagents
Citations for Glo Substrate Reagent Pack
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Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis
Authors: M Inoue, R Nakashima, M Enomoto, Y Koike, X Zhao, K Yip, SH Huang, JN Waldron, M Ikura, FF Liu, SV Bratman
Nat Commun, 2018;9(1):5116. 2018
First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2010;33(3):664-9. 2010
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010;5(2):e9246. 2010
Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
Nutrients, 0;8(11):. 0
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