Glo Substrate Reagent Pack

Reagent A & B
Catalog # Availability Size / Price Qty
DY993
Ancillary Products Available

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Glo Substrate Reagent Pack
1 Image
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Glo Substrate Reagent Pack Summary

INTENDED USE & DESCRIPTION

Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.

PROCEDURE

This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

You must select a language.

x

Citations for Glo Substrate Reagent Pack

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Pharmacological inhibition of sodium-calcium exchange activates NADPH oxidase and induces infection-independent NETotic cell death
    Authors: M Inoue, M Enomoto, M Yoshimura, T Mizowaki
    Redox Biology, 2021;43(0):101983.  2021
  2. Functional Evaluation of AMD-Associated Risk Variants of Complement Factor B
    Authors: C Pilotti, J Greenwood, SE Moss
    Invest. Ophthalmol. Vis. Sci., 2020;61(5):19.  2020
  3. Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis
    Authors: M Inoue, R Nakashima, M Enomoto, Y Koike, X Zhao, K Yip, SH Huang, JN Waldron, M Ikura, FF Liu, SV Bratman
    Nat Commun, 2018;9(1):5116.  2018
  4. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9.  2010
  5. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.  2010
  6. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
    Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
    Nutrients, 0;8(11):.  0

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

DuoSet Ancillary Reagent Kits

Supplemental ELISA Products

Reviews for Glo Substrate Reagent Pack

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Glo Substrate Reagent Pack?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Glo Substrate Reagent Pack
By Leslie Priddy on 04/03/2018

Glo Substrate Reagent Pack (Reagent A & B)
By Eric Sanchez on 06/02/2016