Glo Substrate Reagent Pack

Reagent A & B
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Glo Substrate Reagent Pack
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Citations (6)
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Glo Substrate Reagent Pack Summary


Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.


Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.


This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes. 


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Citations for Glo Substrate Reagent Pack

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Pharmacological inhibition of sodium-calcium exchange activates NADPH oxidase and induces infection-independent NETotic cell death
    Authors: M Inoue, M Enomoto, M Yoshimura, T Mizowaki
    Redox Biology, 2021-04-26;43(0):101983.  2021-04-26
  2. Functional Evaluation of AMD-Associated Risk Variants of Complement Factor B
    Authors: C Pilotti, J Greenwood, SE Moss
    Invest. Ophthalmol. Vis. Sci., 2020-05-11;61(5):19.  2020-05-11
  3. Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis
    Authors: M Inoue, R Nakashima, M Enomoto, Y Koike, X Zhao, K Yip, SH Huang, JN Waldron, M Ikura, FF Liu, SV Bratman
    Nat Commun, 2018-11-30;9(1):5116.  2018-11-30
  4. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution
    Authors: Foteini Kakulas
    Nutrients, 2016-11-08;8(11):.  2016-11-08
  5. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.  2010-02-16
  6. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2009-12-10;33(3):664-9.  2009-12-10


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Glo Substrate Reagent Pack
By Leslie Priddy on 04/03/2018

Glo Substrate Reagent Pack (Reagent A & B)
By Eric Sanchez on 06/02/2016