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Glo Substrate Reagent Pack

R&D Systems | Catalog # DY993

Reagent A & B
R&D Systems
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Key Product Details

Applications

ELISA

Product Summary for Glo Substrate Reagent Pack

INTENDED USE & DESCRIPTION

Glo Substrate Reagent Pack contains 5 vials of Glo Reagent A (stabilized enhanced luminol, Part # 895868, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, Part # 895869, 8 mL/vial) for use in chemiluminescent immunoassay development.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Use within 15 minutes to 4 hours of mixing.

PROCEDURE

This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs. Use black microplates for chemiluminescent assays (Catalog # DY991). Mix 1 part Glo Reagent A to 2 parts Glo Reagent B in a plastic container, cap, and protect from light. Add 100 µL of the mixture to each well and incubate at room temperature while protecting from light. Read the microplate on a luminometer within 5-20 minutes. 

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Glo Substrate Reagent Pack

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Glo Substrate Reagent Pack

For research use only

Citations for Glo Substrate Reagent Pack

Customer Reviews for Glo Substrate Reagent Pack (2)

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  • Glo Substrate Reagent Pack
    Name: Leslie Priddy
    Verified Customer | Posted 04/03/2018
  • Glo Substrate Reagent Pack (Reagent A & B)
    Name: Eric Sanchez
    Verified Customer | Posted 06/02/2016

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