SURF5 Antibody (PCRP-MED22-2A7)
Novus Biologicals | Catalog # NBP3-13788
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-MED22-2A7
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Product Specifications
Immunogen
Recombinant full-length human SURF5 protein (Uniprot: Q15528)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14191)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for SURF5 Antibody (PCRP-MED22-2A7)
![Immunocytochemistry/ Immunofluorescence: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]](https://resources.rndsystems.com/images/products/SURF5-Antibody-PCRP-MED22-2A7-Immunocytochemistry-Immunofluorescence-NBP3-13788-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]")
Immunocytochemistry/ Immunofluorescence: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]
Immunocytochemistry/Immunofluorescence: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using SURF5 Antibody (PCRP-MED22-2A7) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.![Flow Cytometry: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]](https://resources.rndsystems.com/images/products/SURF5-Antibody-PCRP-MED22-2A7-Flow-Cytometry-NBP3-13788-img0001.jpg "Flow Cytometry: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]")
Flow Cytometry: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]
Flow Cytometry: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788] - Flow cytometric analysis of PFA-fixed U87 cells. SURF5 antibody (PCRP-MED22-2A7) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).![Protein Array: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]](https://resources.rndsystems.com/images/products/SURF5-Antibody-PCRP-MED22-2A7-Protein-Array-NBP3-13788-img0004.jpg "Protein Array: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]")
Protein Array: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788]
Protein Array: SURF5 Antibody (PCRP-MED22-2A7) [NBP3-13788] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SURF5 Antibody (PCRP-MED22-2A7).Applications for SURF5 Antibody (PCRP-MED22-2A7)
Application
Recommended Usage
Flow Cytometry
0.5-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SURF5
Alternate Names
MED24, mediator complex subunit 22SURF5surfeit 5, mediator of RNA polymerase II transcription subunit 22, MGC48682, Surf-5, Surfeit locus protein 5
Gene Symbol
MED22
UniProt
Additional SURF5 Products
Product Documents for SURF5 Antibody (PCRP-MED22-2A7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SURF5 Antibody (PCRP-MED22-2A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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