Survivin [p Thr34] Antibody - BSA Free

Novus Biologicals | Catalog # NB500-236

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (94%), Canine (94%), Feline (100%), Porcine (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Survivin Primary Antibodies »

Product Specifications

Immunogen

This Survivin [p Thr34] Antibody was developed against a synthetic peptide with a phosphorylated Threonine (amino acid 34) corresponding to human Survivin. [UniProt# O15392]

Reactivity Notes

Human and mouse reactivity reported in scientific literature (PMID: 17510430).

Modification

p Thr34

Localization

Nuclear

Specificity

Survivin [p Thr34] Antibody [NB500-236] is specific for phosphorylated survivin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin [p Thr34] Antibody - BSA Free

Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.
Immunocytochemistry/Immunofluorescence: Survivin [p Thr34] Antibody [NB500-236] - Immunocytochemical analysis using Survivin [p Thr34] Antibody [NB500-236] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Western Blot: Survivin [p Thr34] Antibody [NB500-236] -

nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-2552023151499.jpg
Survivin [p Thr34] Antibody

Western Blot: Survivin [p Thr34] Antibody [NB500-236] -

Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs & IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. A HE staining & IHC analysis of p-H2AX, c-caspase-3, ki67, PCNA, snail/Slug, & vimentin in the indicated groups. Scale bar = 50 μm. n=5. B Western blot for expression of p-H2AX, c-caspase-3, p-survivin, snail/Slug, & vimentin in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. C In vivo apoptosis marker TUNEL assay in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. n=5. Data are shown as the mean ± standard deviation. ns, not significant. ****p < 0.0001. Scale bar = 200 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Survivin [p Thr34] Antibody - BSA Free

Survivin [p Thr34] in HeLa Human Cell Line.

Survivin [p Thr34] was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin [p Thr34] Antibody - BSA Free

Survivin [p Thr34] in NIH-3T3 Mouse Cell Line.

Survivin [p Thr34] was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells in various stages of mitosis were imaged using a 100X objective and digitally deconvolved.

Applications for Survivin [p Thr34] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 23549285)

Western Blot

1:1000. Use reported in scientific literature (PMID 25298395)
Application Notes
This antibody has been limited to the detection of phosphorylated Survivin recombinant protein and has not yet been successfully used to detect endogenous phosphorylated Survivin.

Reviewed Applications

Read 1 review rated 4 using NB500-236 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Survivin [p Thr34] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Survivin [p Thr34] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Survivin [p Thr34] Antibody - BSA Free

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    Application: Western Blot
    Sample Tested: Cancer Cells
    Species: Mouse
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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Survivin [p Thr34] Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does [p Thr34] in the product name mean?

    A: p Thr34 is a modification; this antibody was developed against a synthetic peptide with a phosphorylated Threonine (amino acid 34) (hence, p Thr34).

  • Q: What does it mean in the predicted species "Porcine (94%)"?

    A: This means that the amino acid sequence used as an immunogen for this antibody is 94% homologous in porcine and can therefore react with this species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this survivin antibody is nuclear.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does [p Thr34] in the product name mean?

    A: p Thr34 is a modification; this antibody was developed against a synthetic peptide with a phosphorylated Threonine (amino acid 34) (hence, p Thr34).

  • Q: What does it mean in the predicted species "Porcine (94%)"?

    A: This means that the amino acid sequence used as an immunogen for this antibody is 94% homologous in porcine and can therefore react with this species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this survivin antibody is nuclear.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does [p Thr34] in the product name mean?

    A: p Thr34 is a modification; this antibody was developed against a synthetic peptide with a phosphorylated Threonine (amino acid 34) (hence, p Thr34).

  • Q: What does it mean in the predicted species "Porcine (94%)"?

    A: This means that the amino acid sequence used as an immunogen for this antibody is 94% homologous in porcine and can therefore react with this species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this survivin antibody is nuclear.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does [p Thr34] in the product name mean?

    A: p Thr34 is a modification; this antibody was developed against a synthetic peptide with a phosphorylated Threonine (amino acid 34) (hence, p Thr34).

  • Q: What does it mean in the predicted species "Porcine (94%)"?

    A: This means that the amino acid sequence used as an immunogen for this antibody is 94% homologous in porcine and can therefore react with this species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this survivin antibody is nuclear.

Showing  1 - 4 of 4 FAQs Showing All
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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail