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Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence CKCGAVTCRGYLN corresponding to C-Terminus according to NP_078946.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SUV39H2 Antibody
Western Blot: SUV39H2 Antibody [NB100-1140]
Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry: SUV39H2 Antibody [NB100-1140]
Immunocytochemistry: SUV39H2 Antibody [NB100-1140] - Dermal Microvascular Endothelial Cells were fixed with 4% Paraformaldehyde followed by immunofluorescence staining using 1:100 dilution of pAb. This image was submitted via customer Review.Applications for SUV39H2 Antibody
Application
Recommended Usage
Peptide ELISA
detection limit 1:8000
Western Blot
1-3 ug/ml
Application Notes
ChIP: An anonymous customer has reported it to work very well. IP per custumer review. ICC per customer review. WB: Approx. 38 kDa band observed in rat kidney and testis lysates (calculated MW of 39.9 kDa band according to NP_078946.1).Preliminary testing was unsuccessful on Mouse, Human and Pig Testis and HEK293 cell line for this particular batch. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31682228).
Reviewed Applications
Read 3 reviews rated 3 using NB100-1140 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SUV39H2
Alternate Names
FLJ23414Histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, histone-lysine N-methyltransferase SUV39H2, KMT1BEC 2.1.1.43, Lysine N-methyltransferase 1B, Su(var)3-9 homolog 2, Suppressor of variegation 3-9 homolog 2, suppressor of variegation 3-9 homolog 2 (Drosophila)
Entrez Gene IDs
79723 (Human)
Gene Symbol
SUV39H2
UniProt
Additional SUV39H2 Products
Product Documents for SUV39H2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SUV39H2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SUV39H2 Antibody
Customer Reviews for SUV39H2 Antibody (3)
3 out of 5
3 Customer Ratings
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Application: ImmunoprecipitationSample Tested: RL human non-Hodgkin's lymphoma B cell lineSpecies: HumanVerified Customer | Posted 05/16/2018IP was performed on human non-Hodgkin's lymphoma B cell line using NB100-1140 and WB was also performed with NB100-1140.
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Application: ImmunocytochemistrySample Tested: Human Dermal Microvascular Endothelial cells - fixed cells used in In-cell ELISA and Human Dermal Microvascular Endothelial Cells - Paraformaldehyde FixedSpecies: HumanVerified Customer | Posted 07/11/2017Human Dermal Microvascular Endothelial Cells were fixed with 4% Paraformaldehyde followed by Immunofluorescence staining using 1:100 dilution of NB100-1140.
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Application: Western BlotSample Tested: Human Dermal Microvascular Endothelial whole cell lysateSpecies: HumanVerified Customer | Posted 05/10/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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