SWAP70 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82979

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SWAP70 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SWAP70 Antibody - BSA Free

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: SWAP70 Antibody [NBP1-82979]

Immunohistochemistry-Paraffin: SWAP70 Antibody [NBP1-82979]

Immunohistochemistry-Paraffin: SWAP70 Antibody [NBP1-82979] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Applications for SWAP70 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-82979 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SWAP70

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion

Long Name

Switch-associated Protein 70 kDa

Alternate Names

KIAA0640SWAP-70FLJ39540, SWAP switching B-cell complex 70kDa subunit, switch-associated protein 70

Gene Symbol

SWAP70

Additional SWAP70 Products

Product Documents for SWAP70 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SWAP70 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation

Citations for SWAP70 Antibody - BSA Free

Customer Reviews for SWAP70 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • SWAP70 Antibody
    Name: Maksim Baranov
    Application: Immunocytochemistry
    Sample Tested: Blood-derived monocytes and Raw 246.7 cells
    Species: Mouse and Human
    Verified Customer | Posted 11/23/2016
    Human dendritic cells were pulsed with FITC-labeled zymosan (zymosan-FITC; green in merge)(pink arrowheads) and latex beads (yellow arrowhead) followed by immunostaining for SWAP70 (magenta). Scale bars, 10 μm.
    For immunocytochemistry the 1:200 dilution was used For Western Blot the 1:500 dilution was used
    SWAP70 Antibody - BSA Free NBP1-82979

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