SYK Antibody (SYK-01) - BSA Free

Novus Biologicals | Catalog # NBP3-09107

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SYK-01

Format

BSA Free
View all SYK Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (aa 5-360) of human Syk.

Specificity

The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SYK Antibody (SYK-01) - BSA Free

Western Blot: SYK Antibody (SYK-01)BSA Free [NBP3-09107]

Western Blot: SYK Antibody (SYK-01)BSA Free [NBP3-09107]

Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control
Immunocytochemistry/ Immunofluorescence: SYK Antibody (SYK-01) - BSA Free [NBP3-09107]

Immunocytochemistry/ Immunofluorescence: SYK Antibody (SYK-01) - BSA Free [NBP3-09107]

Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: SYK Antibody (SYK-01) - BSA Free [NBP3-09107]

Immunocytochemistry/ Immunofluorescence: SYK Antibody (SYK-01) - BSA Free [NBP3-09107]

Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Applications for SYK Antibody (SYK-01) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SYK

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Long Name

Spleen Tyrosine Kinase

Alternate Names

DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK

Gene Symbol

SYK

Additional SYK Products

Product Documents for SYK Antibody (SYK-01) - BSA Free

Certificate of Analysis

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Product Specific Notices for SYK Antibody (SYK-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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