Synapsin I Antibody (7B1D9) - BSA Free
Novus Biologicals | Catalog # NBP2-61894
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7B1D9
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Synapsin I (AA: 362-511) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
74.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Synapsin I Antibody (7B1D9) - BSA Free
Western Blot: Synapsin I Antibody (7B1D9)BSA Free [NBP2-61894]
Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7B1D9) - BSA Free [NBP2-61894]
Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.Western Blot: Synapsin I Antibody (7B1D9)BSA Free [NBP2-61894]
Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)Western Blot: Synapsin I Antibody (7B1D9)BSA Free [NBP2-61894]
Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mouse mAb against NIH/3T3 (1), U251 (2), C6 (3), A549 (4), and MCF-7 (5) cell lysate.Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7B1D9) - BSA Free [NBP2-61894]
Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7B1D9) - BSA Free [NBP2-61894]
Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7B1D9) - BSA Free [NBP2-61894]
Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.ELISA: Synapsin I Antibody (7B1D9) - BSA Free [NBP2-61894]
ELISA: Synapsin I Antibody (7B1D9) [NBP2-61894] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Applications for Synapsin I Antibody (7B1D9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synapsin I
Alternate Names
SYN1
Gene Symbol
SYN1
Additional Synapsin I Products
Product Documents for Synapsin I Antibody (7B1D9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Synapsin I Antibody (7B1D9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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