Synapsin I Pan Specific Antibody

R&D Systems | Catalog # PPS035

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunofluorescence, Immunoprecipitation

Cited:

Reverse Phase Protein Array

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Synapsin I Primary Antibodies »

Product Specifications

Immunogen

Synapsin I

Specificity

This antibody is specific for the ~78 kDa Synapsin I doublet in Western blots of rat brain lysates. Immunolabeling is blocked by preadsorption of the antibody with the native protein immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synapsin I Pan Specific Antibody

Detection of Synapsin I antibody by Western Blot.

Detection of Synapsin I by Western Blot.

Western blot of 10 μg of rat hippocampus lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.

Applications for Synapsin I Pan Specific Antibody

Application
Recommended Usage

Immunofluorescence

1:2000 dilution

Immunohistochemistry

1:2000 dilution

Immunoprecipitation

0.5 µg/100 µg cell lysate

Western Blot

1:1000 dilution
Sample: Rat hippocampus lysate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4) before use.

Formulation

10 μg per vial; lyophilized in 5 mM ammonium bicarbonate.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

The lyophilized product is stable at  ≤ ‑20° C for at least one year. After reconstitution the antibody should be stored at  ≤ ‑20° C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in pre‑synaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces Synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat Synapsin I is 704 amino acids (aa) in length and contains five domains, named A-E/F. Domains A‑D lie between amino acids 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and S566 in domain D, and S9 in domain A, reduces the affinity of Synapsin I for actin and secretory vesicles.

References

  1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
  2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
  3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
  4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
  5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.

Alternate Names

SYN1

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat)

Gene Symbol

SYN1

Additional Synapsin I Products

Product Documents for Synapsin I Pan Specific Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Synapsin I Pan Specific Antibody

For research use only

Related Research Areas

Neuronal Markers

Citations for Synapsin I Pan Specific Antibody

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Protocols

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