Synapsin I Pan Specific Antibody PPS035: R&D Systems

Synapsin I Pan Specific Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Bovine
  • Specificity
    This antibody is specific for the ~78 kDa Synapsin I doublet in Western blots of rat brain lysates. Immunolabeling is blocked by preadsorption of the antibody with the native protein immunogen.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Synapsin I
  • Formulation
    10 μg per vial; lyophilized in 5 mM ammonium bicarbonate.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunohistochemistry
    1:2000 dilution
  • Immunoprecipitation
    0.5 µg/100 µg cell lysate
  • Immunofluorescence
    1:2000 dilution
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Synapsin I by Western Blot. Western blot of 10 μg of rat hippocampus lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.
Preparation and Storage
  • Reconstitution
    This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4) before use.
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    The lyophilized product is stable at  ≤ ‑20° C for at least one year. After reconstitution the antibody should be stored at  ≤ ‑20° C.
Background: Synapsin I
Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in pre‑synaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces Synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat Synapsin I is 704 amino acids (aa) in length and contains five domains, named A-E/F. Domains A‑D lie between amino acids 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and S566 in domain D, and S9 in domain A, reduces the affinity of Synapsin I for actin and secretory vesicles.
  • References:
    1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
    2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
    3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
    4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
    5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.
  • Entrez Gene IDs:
    6853 (Human); 20964 (Mouse); 24949 (Rat)
  • Alternate Names:
    SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin I; synapsin IBrain protein 4.1; synapsin-1; SYNI
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