Detection of Synapsin I by Western Blot. Western blot of 10 μg of rat hippocampus lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.
Preparation and Storage
antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4,
2.7 mM KCl, 1.5 mM KH2PO4) before use.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
The lyophilized product is stable at ≤ ‑20° C
for at least one year. After reconstitution the antibody should be stored at ≤ ‑20° C.
Background: Synapsin I
I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts
with synaptic vesicles and regulates neurotransmitter release. It is
concentrated in pre‑synaptic terminals where it anchors synaptic vesicles to
the underlying cytoskeleton. Phosphorylation at various sites reduces Synapsin
I binding to neurosecretory vesicles, making them available for
neurotransmitter release. Rat Synapsin I is 704 amino acids (aa) in length and
contains five domains, named A-E/F. Domains A‑D lie between amino
acids 1-655, while E and F are alternate splice forms. Domain A (28 aa)
regulates neurotransmitter release in a phosphorylation-dependent manner. Domains
C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84
aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin
nucleation. Phosphorylation of S603 and S566 in domain D, and S9 in domain A,
reduces the affinity of Synapsin I for actin and secretory vesicles.
Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.
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