Synaptotagmin 2 Antibody

Immunohistochemistry: Synaptotagmin 2 Antibody [NB100-91297]

Immunohistochemistry: Synaptotagmin 2 Antibody [NB100-91297] - IHC on rat spinal cord using Rabbit antibody to rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2): whole serum at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Immunohistochemistry: Synaptotagmin 2 Antibody [NB100-91297]

Immunohistochemistry: Synaptotagmin 2 Antibody [NB100-91297] - IHC on rat spinal cord using Rabbit antibody to rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2): whole serum at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Western Blot: Synaptotagmin 2 Antibody [NB100-91297] -

Restoration of Otoferlin levels attenuates the cognitive deficits and membrane trafficking protein perturbations observed after Slc20a1 downregulation.A Relative expression of Slc20a1 and Otof in hippocampi injected with either shRNA-Scramble, shRNA-Slc20a1, or shRNA- Slc20a1+ shRNA-Otof. B Western blot analyses of Otof, Syt2 and Rab5 in hippocampi from Scramble (n = 4), shRNA-Slc20a1 (n = 4), shRNA-Otof (n = 5) or shRNA-Slc20a1 + shRNA-Otof (n = 5) mice. beta -actin was used as a loading control for each sample. C Relative GABA measurements performed in Scramble, shRNA-Slc20a1 or shRNA-Slc20a1 + shRNA-Otof. D–E Morris Water Maze (D) and Novel Object Recognition (NOR) (E) was performed in 3-month-old mice injected with either Scramble, shRNA-Slc20a1 or shRNA-Slc20a1 + shRNA-Otof. FGabrR2, Kcnj10, Syt2 and Syt1 relative expression in hippocampi from Scramble, shRNA-Slc20a1 or shRNA-Slc20a1 + shRNA-Otof - injected mice. All behavioral tests were performed using at least 2 independent animal cohorts. G Western blot analyses of Otof, GABAR2, and the total and phosphorylated form of GluA1 (Ser845 and 831) in HpC injected with either Scramble, shRNA-Slc20a1, shRNA-Otof or shRNA-Slc20a1 + shRNA-Otof. GAPDH was used as a loading control for each sample. Results are given as mean +/- s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 by two-tailed Student’s t-test or 2-way ANOVA followed by Tukey’s multiple comparisons test compared to the control group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-023-06292-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Synaptotagmin 2 Antibody [NB100-91297] -

PiT-1 promotes synaptic vesicle fusion and membrane trafficking in hippocampal neurons.A Synaptotagmin 2 (Syt2), Synaptotagmin 1 (Syt1) and Otoferlin (Otof) relative gene expression in the HpC after local stereotactic injections of either Scramble or shRNA-Slc20a1. B Western Blot analyses of Syt2, Rab5 and Otof in HpC after injection of AAVs expressing either Scramble or shRNA-Slc20a1. beta -actin was used as a loading control for each sample. C Otorferlin, GABAR2, Syt2 and EAA1 immunofluorescence (scale bar = 50 μm) on the HpC CA3 region (n = 5) after Slc20a1 silencing and compared to controls. Brains were collected from two independent cohorts of mice for each group. Results are given as mean +/- s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p < 0.0001, NS: not significant by two-tailed Student’s t-test compared to control group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-023-06292-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.