Syntaxin 4 Antibody (6D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00006810-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6D1

Format

Azide and BSA Free
View all Syntaxin 4 Primary Antibodies »

Product Specifications

Immunogen

STX4A (NP_004595, 19 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVN

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

STX4A (6D1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Syntaxin 4 Antibody (6D1) - Azide and BSA Free

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of monoclonal antibody to STX4A on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in HeLa (Cat # L013V1).
Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in Raw 264.7 (Cat # L024V1).
Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04]

Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4 expression in transfected 293T cell line by STX4A monoclonal antibody (M04), clone 6D1. Lane 1: STX4 transfected lysate (Predicted MW: 34.2 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Detection limit for recombinant GST tagged STX4A is approximately 0.1ng/ml as a capture antibody.

Applications for Syntaxin 4 Antibody (6D1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Syntaxin 4

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. May also play a role in docking of synaptic vesicles at presynaptic active zones

Alternate Names

NY-REN-31, p35-2, STX4

Entrez Gene IDs

6810 (Human)

Gene Symbol

STX4

OMIM

186591 (Human)

UniProt

NP_004595 (Human)

Additional Syntaxin 4 Products

Product Documents for Syntaxin 4 Antibody (6D1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Syntaxin 4 Antibody (6D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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