Syntaxin-BP1 [p Ser513] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-742

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Syntaxin-BP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 conjugated to KLH. Accession # Q62753

Modification

p Ser513

Specificity

Specific for endogenous levels of the ~65 kDa Syntaxin-BP1 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syntaxin-BP1 [p Ser513] Antibody - Azide Free

Western Blot: Syntaxin-BP1 [p Ser513] Antibody [NB300-742]

Western Blot: Syntaxin-BP1 [p Ser513] Antibody [NB300-742]

Western Blot: Syntaxin-BP1 [p Ser515] Antibody [NB300-742] - Rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
Immunohistochemistry: Syntaxin-BP1 [p Ser513] Antibody [NB300-742]

Immunohistochemistry: Syntaxin-BP1 [p Ser513] Antibody [NB300-742]

Immunohistochemistry: Syntaxin-BP1 [p Ser513] Antibody [NB300-742] - Immunostaining of 14 DIV rat cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser513 (red) and PSD95 (green). Photo courtesy of Gang Liu.

Applications for Syntaxin-BP1 [p Ser513] Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:400

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntaxin-BP1

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part to its ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).

Long Name

Syntaxin-binding Protein 1

Alternate Names

EIEE4, p67, rbSec1, STXBP1, SyntaxinBP1, UNC18

Entrez Gene IDs

25558 (Rat)

Gene Symbol

STXBP1

UniProt

Q62753 (Rat)

Additional Syntaxin-BP1 Products

Product Documents for Syntaxin-BP1 [p Ser513] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Syntaxin-BP1 [p Ser513] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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