Syntaxin-BP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83139

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syntaxin-BP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Analysis in human cerebral cortex and liver tissues using NBP1-83139 antibody. Corresponding STXBP1 RNA-seq data are presented for the same tissues.
Western Blot: Syntaxin-BP1 Antibody [NBP1-83139]

Western Blot: Syntaxin-BP1 Antibody [NBP1-83139]

Western Blot: Syntaxin-BP1 Antibody [NBP1-83139] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139]

Immunocytochemistry/ Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139]

Immunocytochemistry/Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human cerebellum shows strong positivity in molecular layer.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139]

Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Syntaxin-BP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin-BP1

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part to its ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).

Long Name

Syntaxin-binding Protein 1

Alternate Names

EIEE4, p67, rbSec1, STXBP1, SyntaxinBP1, UNC18

Gene Symbol

STXBP1

Additional Syntaxin-BP1 Products

Product Documents for Syntaxin-BP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Syntaxin-BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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