Syntrophin Antibody (1351)
Novus Biologicals | Catalog # NB600-1294
![Western Blot: Syntrophin Antibody (1351) [NB600-1294]](https://resources.rndsystems.com/images/products/Syntrophin-Antibody-1351-Western-Blot-NB600-1294-img0016.jpg "Western Blot: Syntrophin Antibody (1351) [NB600-1294]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Canine, Chicken
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1351
Loading...
Product Specifications
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Epitope
Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Neuromuscular synapse
Specificity
This antibody detects the alpha 1, beta 1 and beta 2 subunits of Syntrophin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Syntrophin Antibody (1351)
Western Blot: Syntrophin Antibody (1351) [NB600-1294]
Western Blot: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin on canine lysate.![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.rndsystems.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0019.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]
Immunocytochemistry/Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin using Syntrophin Monoclonal Antibody (1351) shows staining in Hela Cells. Syntrophin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Syntrophin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.rndsystems.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0018.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]
Immunocytochemistry/Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin using Syntrophin Monoclonal Antibody (1351) shows staining in 293Cells. Syntrophin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Syntrophin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.rndsystems.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0017.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]
Immunocytochemistry/Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin using Syntrophin Monoclonal Antibody (1351) shows staining in A2058 Cells. Syntrophin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Syntrophin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Applications for Syntrophin Antibody (1351)
Application
Recommended Usage
Flow Cytometry
0.5 ug / 10^6 cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
0.1 - 1 ug/ml
Application Notes
WB: Detects an approx. 58 kDa protein representing syntrophin from rat and mouse cell extracts.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Syntrophin
Alternate Names
59 kDa dystrophin-associated protein A1 acidic component 1, dJ1187J4.5, LQT12, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, SNT1alpha-1-syntrophin, syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component), syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component), syntrophin-1, TACIP1acidic alpha 1 syntrophin
Gene Symbol
SNTA1
UniProt
Additional Syntrophin Products
Product Documents for Syntrophin Antibody (1351)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Syntrophin Antibody (1351)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Syntrophin Antibody (1351)
Powered by Bioz
Customer Reviews for Syntrophin Antibody (1351)
There are currently no reviews for this product. Be the first to review Syntrophin Antibody (1351) and earn rewards!
Have you used Syntrophin Antibody (1351)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...