t-Plasminogen Activator/tPA Antibody

Novus Biologicals | Catalog # NBP2-20648

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all t-Plasminogen Activator/tPA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human t-Plasminogen Activator/tPA. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (82%), Bovine (82%).

Localization

Secreted; extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for t-Plasminogen Activator/tPA Antibody

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Colon carcinoma. tPA antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648]

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human hepatoma. tPA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
t-Plasminogen Activator/tPA Antibody

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] -

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with t-Plasminogen Activator/tPA antibody (NBP2-20648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
t-Plasminogen Activator/tPA Antibody

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] -

Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (NBP2-20648) diluted at 1:2000.
t-Plasminogen Activator/tPA Antibody

Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] -

tPA antibody detects PLAT protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (NBP2-20648) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for t-Plasminogen Activator/tPA Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: t-Plasminogen Activator/tPA

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Tissue Plasminogen Activator

Alternate Names

Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator

Gene Symbol

PLAT

UniProt

P00750

Additional t-Plasminogen Activator/tPA Products

Product Documents for t-Plasminogen Activator/tPA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for t-Plasminogen Activator/tPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for t-Plasminogen Activator/tPA Antibody

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Protocols

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FAQs

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