T Plastin Antibody (5B9) - Azide and BSA Free

Novus Biologicals | Catalog # H00005358-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B9

Format

Azide and BSA Free
View all T Plastin Primary Antibodies »

Product Specifications

Immunogen

PLS3 (AAH39049, 1 a.a. ~ 630 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDGKISFDEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV

Specificity

This product is specific for Human PLS3 monoclonal antibody (M01), clone 5B9 [Gene ID: 5358].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for T Plastin Antibody (5B9) - Azide and BSA Free

Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - Analysis of PLS3 expression in transfected 293T cell line by PLS3 monoclonal antibody (M01), clone 5B9.Lane 1: PLS3 transfected lysate (Predicted MW: 70.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: T Plastin Antibody (5B9) [H00005358-M01]

Immunocytochemistry/ Immunofluorescence: T Plastin Antibody (5B9) [H00005358-M01]

Immunocytochemistry/Immunofluorescence: T Plastin Antibody (5B9) [H00005358-M01] - Analysis of monoclonal antibody to PLS3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - PLS3 monoclonal antibody (M01), clone 5B9. Analysis of PLS3 expression in MCF-7.
Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01]

Western Blot: T Plastin Antibody (5B9) [H00005358-M01] - PLS3 monoclonal antibody (M01), clone 5B9. Analysis of PLS3 expression in MDA-MB-231.
ELISA: T Plastin Antibody (5B9) [H00005358-M01]

ELISA: T Plastin Antibody (5B9) [H00005358-M01]

ELISA: T Plastin Antibody (5B9) [H00005358-M01] - Detection limit for recombinant GST tagged PLS3 is 0.1 ng/ml as a capture antibody.

Applications for T Plastin Antibody (5B9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: T Plastin

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

plastin 3, plastin 3 (T isoform), plastin-3, T plastin, T-plastinT fimbrin

Gene Symbol

PLS3

UniProt

AAH39049 (Human)

Additional T Plastin Products

Product Documents for T Plastin Antibody (5B9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for T Plastin Antibody (5B9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for T Plastin Antibody (5B9) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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