T Plastin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13778

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all T Plastin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: PAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPGAPHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for T Plastin Antibody - BSA Free

Western Blot: T Plastin Antibody [NBP2-13778]

Western Blot: T Plastin Antibody [NBP2-13778]

Western Blot: T Plastin Antibody [NBP2-13778] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-565
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778] - Staining of human small intestine shows low expression as expected.
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778] - Staining of human seminal vesicle shows high expression.
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP2-13778] - Staining in human seminal vesicle and small intestine tissues using anti-PLS3 antibody. Corresponding PLS3 RNA-seq data are presented for the same tissues.

Applications for T Plastin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: T Plastin

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

plastin 3, plastin 3 (T isoform), plastin-3, T plastin, T-plastinT fimbrin

Gene Symbol

PLS3

Additional T Plastin Products

Product Documents for T Plastin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for T Plastin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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