TACS 2 TdT-DAB In Situ Apoptosis Detection Kit
TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary
• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.
Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?
The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.
This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.
- In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
- Light microscopy.
• Proteinase K
• DAB Enhancer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
- Proteinase K
- DAB Enhancer
- TACS 2 TdT dNTP
- TdT Enzyme
- 50x Cobalt Cation
- 50x Magnesium Cation
- 50x Manganese Cation
- TACS-Nuclease™ Buffer
- Methyl Green 1%
- TACS 2 TdT Labeling Buffer
- TACS 2 TdT Stop Buffer
For research use only. Not for diagnositic use.
No product specific FAQs exist for this product, however you mayView all FAQs
Reviews for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit
There are currently no reviews for this product. Be the first to review TACS 2 TdT-DAB In Situ Apoptosis Detection Kit and earn rewards!
Have you used TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image