TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-K
Product Details
Citations (74)
FAQs
Reviews (1)

TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Provides a unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

74 Citations: Showing 1 - 10
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  1. MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis
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  22. Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines
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    FASEB J., 2018;0(0):fj201701176R.  2018
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    Res. Vet. Sci., 2017;112(0):141-148.  2017
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    Mol Med Rep, 2017;16(4):4437-4444.  2017
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    Am J Pathol, 2016;186(1):87-100.  2016
  34. PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma
    PLoS Genet, 2016;12(12):e1006518.  2016
  35. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma
    Onco Targets Ther, 2016;9(0):4023-35.  2016
  36. FBXW7 acts as an independent prognostic marker and inhibits tumor growth in human osteosarcoma.
    Authors: Li, Zhanchun, Xiao, Jie, Hu, Kongzu, Wang, Gang, Li, Maoqiang, Zhang, Jidong, Cheng, Guangqi
    Int J Mol Sci, 2015;16(2):2294-306.  2015
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  70. Acceleration of diabetic wound healing using a novel protease-anti-protease combination therapy.
    Authors: Gao M, Nguyen T, Suckow M, Wolter W, Gooyit M, Mobashery S, Chang M
    Proc Natl Acad Sci U S A, 0;112(49):15226-31.  0
  71. Transient increase of fractional anisotropy in reversible vasogenic edema.
    Authors: Kimura-Ohba S, Yang Y, Thompson J, Kimura T, Salayandia V, Cosse M, Yang Y, Sillerud L, Rosenberg G
    J Cereb Blood Flow Metab, 0;36(10):1731-1743.  0
  72. Intestinal myofibroblast-specific Tpl2-Cox-2-PGE2 pathway links innate sensing to epithelial homeostasis.
    Authors: Roulis M, Nikolaou C, Kotsaki E, Kaffe E, Karagianni N, Koliaraki V, Salpea K, Ragoussis J, Aidinis V, Martini E, Becker C, Herschman H, Vetrano S, Danese S, Kollias G
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  73. TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord.
    Authors: Church J, Kigerl K, Lerch J, Popovich P, McTigue D
    J Neurosci, 0;36(23):6352-64.  0
  74. Cryopreservation Method for the Effective Collection of Dental Pulp Stem Cells.
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    Tissue Eng Part C Methods, 0;23(5):251-261.  0

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TdT In Situ Apoptosis Detection Kit - DAB
By Anonymous on 10/11/2016
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