TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

• In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
• Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Limitations

For research use only. Not for diagnositic use.

Product Datasheets