TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-K
Product Details
Citations (69)
FAQs
Reviews (1)

TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Provides a unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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  1. Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artificial Vessels Composed of Autologous Mesenchymal Stem Cells
    Authors: EH Jang, JH Kim, JH Lee, DH Kim, YN Youn
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  2. Compensatory combination of romidepsin with gemcitabine and cisplatin to effectively and safely control urothelial carcinoma
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    Br. J. Cancer, 2020;0(0):.  2020
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    Cell Death Dis, 2020;11(1):28.  2020
  5. VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacterial Infection
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    Cancers (Basel), 2019;11(12):.  2019
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  19. X-linked inhibitor of apoptosis inhibition sensitizes acute myeloid leukemia cell response to TRAIL and chemotherapy through potentiated induction of proapoptotic machinery
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  20. Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines
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  21. Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice
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    Front Physiol, 2018;9(0):324.  2018
  22. Role of SIRT2 in Regulation of Stemness of Cancer Stem-Like Cells in Renal Cell Carcinoma
    Authors: R Wei, D He, X Zhang
    Cell. Physiol. Biochem., 2018;49(6):2348-2357.  2018
  23. Treatment of Arsenite Intoxication-Induced Peripheral Vasculopathy with Mesenchymal Stem Cells
    Authors: YH Chiang, CC Lin, YC Chen, OK Lee
    Int J Mol Sci, 2018;19(4):.  2018
  24. Calcium-dependent enhancement by extracellular acidity of the cytotoxicity of mitochondrial inhibitors against melanoma
    Authors: F Noguchi, S Inui, C Fedele, M Shackleton, S Itami
    Mol. Cancer Ther, 2017;0(0):.  2017
  25. Influence of long-term, high-dose dexamethasone administration on proliferation and apoptosis in porcine hepatocytes
    Authors: M Mikiewicz, I Otrocka-Do, K Pa?dzior-C, T Rotkiewicz
    Res. Vet. Sci., 2017;112(0):141-148.  2017
  26. Histone demethylase PHF8 accelerates the progression of colorectal cancer and can be regulated by miR-488 in�vitro
    Authors: Y Lv, Y Shi, Q Han, G Dai
    Mol Med Rep, 2017;16(4):4437-4444.  2017
  27. Chronic PARP-1 inhibition reduces carotid vessel remodeling and oxidative damage of the dorsal hippocampus in spontaneously hypertensive rats
    Authors: K Eros, K Magyar, L Deres, A Skazel, A Riba, Z Vamos, T Kalai, F Gallyas, B Sumegi, K Toth, R Halmosi
    PLoS ONE, 2017;12(3):e0174401.  2017
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  29. PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma
    PLoS Genet, 2016;12(12):e1006518.  2016
  30. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma
    Onco Targets Ther, 2016;9(0):4023-35.  2016
  31. FBXW7 acts as an independent prognostic marker and inhibits tumor growth in human osteosarcoma.
    Authors: Li, Zhanchun, Xiao, Jie, Hu, Kongzu, Wang, Gang, Li, Maoqiang, Zhang, Jidong, Cheng, Guangqi
    Int J Mol Sci, 2015;16(2):2294-306.  2015
  32. Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema.
    Authors: Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y
    J Immunol, 2014;192(11):5059-68.  2014
  33. Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.
    Authors: Park H, Larson B, Kolewe M, Vunjak-Novakovic G, Freed L
    Exp Cell Res, 2014;321(2):297-306.  2014
  34. Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.
    Authors: Tu, Kangshen, Yang, Wei, Li, Chao, Zheng, Xin, Lu, Zhongtan, Guo, Cheng, Yao, Yingmin, Liu, Qingguan
    Mol Cancer, 2014;13(0):110.  2014
  35. Inhibition of JNK phosphorylation by a novel curcumin analog prevents high glucose-induced inflammation and apoptosis in cardiomyocytes and the development of diabetic cardiomyopathy.
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    Diabetes, 2014;63(10):3497-511.  2014
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    Oncol Rep, 2014;32(4):1571-7.  2014
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    PLoS ONE, 2014;9(2):e90146.  2014
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    Vet Res, 2014;45(0):14.  2014
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    Int J Mol Sci, 2013;14(9):17729-43.  2013
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    J Virol, 0;86(23):12665-75.  0
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    Authors: Takebe Y, Tatehara S, Fukushima T, Tokuyama-Toda R, Yasuhara R, Mishima K, Satomura K
    Tissue Eng Part C Methods, 0;23(5):251-261.  0
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    Proc Natl Acad Sci U S A, 0;112(49):15226-31.  0
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  51. IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice.
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    Biol Reprod, 0;92(3):78.  0
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    J Neurosci, 0;36(23):6352-64.  0
  68. Targeted Deletion of p53 in Lgr5-Expressing Intestinal Stem Cells Promotes Colon Tumorigenesis in a Preclinical Model of Colitis-Associated Cancer.
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    J Cereb Blood Flow Metab, 0;36(10):1731-1743.  0

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TdT In Situ Apoptosis Detection Kit - DAB
By Anonymous on 10/11/2016
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