TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-K

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Product Details
Citations (88)
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TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Provides a unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay.
 

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

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Citations for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

88 Citations: Showing 1 - 10
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  1. Developmental basis of evolutionary lung loss in plethodontid salamanders
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    Science Advances, 2022;8(33):eabo6108.  2022
  2. Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control
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    Redox Biology, 2022;51(0):102275.  2022
  3. MSK-Mediated Phosphorylation of Histone H3 Ser28 Couples MAPK Signalling with Early Gene Induction and Cardiac Hypertrophy
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  20. Periadventitial Delivery of Simvastatin-Loaded Microparticles Attenuate Venous Neointimal Hyperplasia Associated With Arteriovenous Fistula
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  22. Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artificial Vessels Composed of Autologous Mesenchymal Stem Cells
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    Cancers (Basel), 2019;11(12):.  2019
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    Cell Rep, 2019;26(4):984-995.e6.  2019
  30. A Simple and Useful Predictive Assay for Evaluating the Quality of Isolated Hepatocytes for Hepatocyte Transplantation
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    Sci Rep, 2019;9(1):6166.  2019
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    Authors: N Haruyama, K Sakumi, A Katogi, D Tsuchimoto, G De Luca, M Bignami, Y Nakabeppu
    Prog. Neurobiol., 2019;0(0):.  2019
  32. Schwannoma gene therapy by adeno-associated virus delivery of the pore-forming protein Gasdermin-D
    Authors: SG Ahmed, A Ahmed, M Doha, GJ Brenner
    Cancer Gene Ther., 2019;0(0):.  2019
  33. VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacterial Infection
    Authors: JS Harding, M Herbath, Y Chen, A Rayasam, A Ritter, B Csoka, G Hasko, IP Michael, Z Fabry, A Nagy, M Sandor
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    J Neuroinflammation, 2018;15(1):315.  2018
  36. Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice
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    Front Physiol, 2018;9(0):324.  2018
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    Cell. Physiol. Biochem., 2018;49(6):2348-2357.  2018
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  79. Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development.
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    Proc Natl Acad Sci U S A, 0;109(22):8699-704.  0
  81. Neonatal uterine and vaginal cell proliferation and adenogenesis are independent of estrogen receptor 1 (ESR1) in the mouse.
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  82. Transient increase of fractional anisotropy in reversible vasogenic edema.
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TdT In Situ Apoptosis Detection Kit - DAB
By Anonymous on 10/11/2016