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TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

4810-30-K 69 Citations 1 Review Datasheet / COA / SDS
In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-K
Product Details
Citations (69)
FAQs
Reviews (1)
Summary Product Datasheets Background

TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Provides a unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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  1. Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artificial Vessels Composed of Autologous Mesenchymal Stem Cells
    Authors: EH Jang, JH Kim, JH Lee, DH Kim, YN Youn
    Polymers (Basel), 2020;12(3):.  2020
  2. Compensatory combination of romidepsin with gemcitabine and cisplatin to effectively and safely control urothelial carcinoma
    Authors: P Pattarawat, T Hong, S Wallace, Y Hu, R Donnell, TH Wang, CL Tsai, J Wang, HR Wang
    Br. J. Cancer, 2020;0(0):.  2020
  3. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis
    Authors: Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya
    Autophagy, 2020;0(0):1-10.  2020
  4. Targeting alkaline ceramidase 3 alleviates the severity of nonalcoholic steatohepatitis by reducing oxidative stress
    Authors: K Wang, C Li, X Lin, H Sun, R Xu, Q Li, Y Wei, Y Li, J Qian, C Liu, Q Zhang, S Yu, Z Cui, X Huang, B Zhu, J Zhou, C Mao
    Cell Death Dis, 2020;11(1):28.  2020
  5. VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacterial Infection
    Authors: JS Harding, M Herbath, Y Chen, A Rayasam, A Ritter, B Csoka, G Hasko, IP Michael, Z Fabry, A Nagy, M Sandor
    Cell Rep, 2019;27(7):2119-2131.e6.  2019
  6. Schwannoma gene therapy by adeno-associated virus delivery of the pore-forming protein Gasdermin-D
    Authors: SG Ahmed, A Ahmed, M Doha, GJ Brenner
    Cancer Gene Ther., 2019;0(0):.  2019
  7. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon
    Authors: S Gurung, N Reuter, A Preno, J Dubaut, H Nadeau, K Hyatt, K Singleton, A Martin, WT Parks, JF Papin, DA Myers
    PLoS Pathog., 2019;15(1):e1007507.  2019
  8. MiR-30c/PGC-1? protects against diabetic cardiomyopathy via PPAR?
    Authors: Z Yin, Y Zhao, M He, H Li, J Fan, X Nie, M Yan, C Chen, DW Wang
    Cardiovasc Diabetol, 2019;18(1):7.  2019
  9. Epithelial ovarian cancer: feasibility of image-guided intratumoral radiofrequency hyperthermia-enhanced direct gene therapy
    Authors: G Jin, Y Li, F Zhang, P Li, L Zhao, Y Zhou, H Ji, S Pietrini, B Zhai, X Yang
    Am J Cancer Res, 2019;9(2):378-389.  2019
  10. Impaired LXR? Phosphorylation Attenuates Progression of Fatty Liver Disease
    Authors: N Becares, MC Gage, M Voisin, E Shrestha, L Martin-Gut, N Liang, R Louie, B Pourcet, OM Pello, TV Luong, S Goñi, C Pichardo-A, H Røberg-Lar, V Diaz-Zucca, KR Steffensen, A O'Brien, MJ Garabedian, K Rombouts, E Treuter, I Pineda-Tor
    Cell Rep, 2019;26(4):984-995.e6.  2019
  11. 8-Oxoguanine accumulation in aged female brain impairs neurogenesis in the dentate gyrus and major island of Calleja, causing sexually dimorphic phenotypes
    Authors: N Haruyama, K Sakumi, A Katogi, D Tsuchimoto, G De Luca, M Bignami, Y Nakabeppu
    Prog. Neurobiol., 2019;0(0):.  2019
  12. A Simple and Useful Predictive Assay for Evaluating the Quality of Isolated Hepatocytes for Hepatocyte Transplantation
    Authors: M Matsumura, T Imura, A Inagaki, H Ogasawara, K Fukuoka, I Fathi, S Miyagi, K Ohashi, M Unno, T Kamei, S Satomi, M Goto
    Sci Rep, 2019;9(1):6166.  2019
  13. Triptolide sensitizes cisplatin-resistant human epithelial ovarian cancer by inhibiting the phosphorylation of AKT
    Authors: G Huang, H Hu, Y Zhang, Y Zhu, J Liu, B Tan, T Chen
    J Cancer, 2019;10(13):3012-3020.  2019
  14. Attenuation of diabetic cardiomyopathy by relying on kirenol to suppress inflammation in a diabetic rat model
    Authors: B Wu, XY Huang, L Li, XH Fan, PC Li, CQ Huang, J Xiao, R Gui, S Wang
    J. Cell. Mol. Med., 2019;0(0):.  2019
  15. Inhibitor of DNA-Binding Protein 4 Suppresses Cancer Metastasis through the Regulation of Epithelial Mesenchymal Transition in Lung Adenocarcinoma
    Authors: CC Wang, YL Hsu, CJ Chang, CJ Wang, TH Hsiao, SH Pan
    Cancers (Basel), 2019;11(12):.  2019
  16. Serum vitamin C levels modulate the lifespan and endoplasmic reticulum stress response pathways in mice synthesizing a nonfunctional mutant WRN protein
    Authors: L Aumailley, MJ Dubois, TA Brennan, C Garand, ER Paquet, RJ Pignolo, A Marette, M Lebel
    FASEB J., 2018;0(0):fj201701176R.  2018
  17. Concomitant modulation of PTEN and Livin in gastric cancer treatment
    Authors: CL Zhao, SN Han, ZJ Wang, SH Wang, GQ Zhao, XF Zhang, JX Wang
    Int. J. Mol. Med., 2018;0(0):.  2018
  18. RIPK3 promotes sepsis-induced acute kidney injury via mitochondrial dysfunction
    Authors: A Sureshbabu, E Patino, KC Ma, K Laursen, EJ Finkelszte, O Akchurin, T Muthukumar, SW Ryter, L Gudas, AMK Choi, ME Choi
    JCI Insight, 2018;3(11):.  2018
  19. X-linked inhibitor of apoptosis inhibition sensitizes acute myeloid leukemia cell response to TRAIL and chemotherapy through potentiated induction of proapoptotic machinery
    Authors: J Zhou, X Lu, TZ Tan, WJ Chng
    Mol Oncol, 2018;12(1):33-47.  2018
  20. Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines
    Authors: BE Dawes, J Gao, C Atkins, JT Nelson, K Johnson, P Wu, AN Freiberg
    J Neuroinflammation, 2018;15(1):315.  2018
  21. Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice
    Authors: G Ruan, H Ren, C Zhang, X Zhu, C Xu, L Wang
    Front Physiol, 2018;9(0):324.  2018
  22. Role of SIRT2 in Regulation of Stemness of Cancer Stem-Like Cells in Renal Cell Carcinoma
    Authors: R Wei, D He, X Zhang
    Cell. Physiol. Biochem., 2018;49(6):2348-2357.  2018
  23. Treatment of Arsenite Intoxication-Induced Peripheral Vasculopathy with Mesenchymal Stem Cells
    Authors: YH Chiang, CC Lin, YC Chen, OK Lee
    Int J Mol Sci, 2018;19(4):.  2018
  24. Calcium-dependent enhancement by extracellular acidity of the cytotoxicity of mitochondrial inhibitors against melanoma
    Authors: F Noguchi, S Inui, C Fedele, M Shackleton, S Itami
    Mol. Cancer Ther, 2017;0(0):.  2017
  25. Influence of long-term, high-dose dexamethasone administration on proliferation and apoptosis in porcine hepatocytes
    Authors: M Mikiewicz, I Otrocka-Do, K Pa?dzior-C, T Rotkiewicz
    Res. Vet. Sci., 2017;112(0):141-148.  2017
  26. Histone demethylase PHF8 accelerates the progression of colorectal cancer and can be regulated by miR-488 in�vitro
    Authors: Y Lv, Y Shi, Q Han, G Dai
    Mol Med Rep, 2017;16(4):4437-4444.  2017
  27. Chronic PARP-1 inhibition reduces carotid vessel remodeling and oxidative damage of the dorsal hippocampus in spontaneously hypertensive rats
    Authors: K Eros, K Magyar, L Deres, A Skazel, A Riba, Z Vamos, T Kalai, F Gallyas, B Sumegi, K Toth, R Halmosi
    PLoS ONE, 2017;12(3):e0174401.  2017
  28. Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
    Authors: Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D
    Am J Pathol, 2016;186(1):87-100.  2016
  29. PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma
    PLoS Genet, 2016;12(12):e1006518.  2016
  30. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma
    Onco Targets Ther, 2016;9(0):4023-35.  2016
  31. FBXW7 acts as an independent prognostic marker and inhibits tumor growth in human osteosarcoma.
    Authors: Li, Zhanchun, Xiao, Jie, Hu, Kongzu, Wang, Gang, Li, Maoqiang, Zhang, Jidong, Cheng, Guangqi
    Int J Mol Sci, 2015;16(2):2294-306.  2015
  32. Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema.
    Authors: Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y
    J Immunol, 2014;192(11):5059-68.  2014
  33. Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.
    Authors: Park H, Larson B, Kolewe M, Vunjak-Novakovic G, Freed L
    Exp Cell Res, 2014;321(2):297-306.  2014
  34. Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.
    Authors: Tu, Kangshen, Yang, Wei, Li, Chao, Zheng, Xin, Lu, Zhongtan, Guo, Cheng, Yao, Yingmin, Liu, Qingguan
    Mol Cancer, 2014;13(0):110.  2014
  35. Inhibition of JNK phosphorylation by a novel curcumin analog prevents high glucose-induced inflammation and apoptosis in cardiomyocytes and the development of diabetic cardiomyopathy.
    Authors: Pan Y, Wang Y, Zhao Y, Peng K, Li W, Wang Y, Zhang J, Zhou S, Liu Q, Li X, Cai L, Liang G
    Diabetes, 2014;63(10):3497-511.  2014
  36. Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth.
    Authors: Tu K, Li C, Zheng X, Yang W, Yao Y, Liu Q
    Oncol Rep, 2014;32(4):1571-7.  2014
  37. Mebendazole reduces vascular smooth muscle cell proliferation and neointimal formation following vascular injury in mice.
    Authors: Wang J, Wang H, Guo C, Luo W, Lawler A, Reddy A, Wang J, Sun E, Eitzman D
    PLoS ONE, 2014;9(2):e90146.  2014
  38. Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD).
    Authors: Marques R, Teixeira L, Aguas A, Ribeiro J, Costa-e-Silva A, Ferreira P
    Vet Res, 2014;45(0):14.  2014
  39. Increased chondrocyte apoptosis is associated with progression of osteoarthritis in spontaneous Guinea pig models of the disease.
    Authors: Zamli Z, Adams M, Tarlton J, Sharif M
    Int J Mol Sci, 2013;14(9):17729-43.  2013
  40. The alternative complement pathway propagates inflammation and injury in murine ischemic stroke.
    Authors: Elvington, Andrew, Atkinson, Carl, Zhu, Hong, Yu, Jin, Takahashi, Kazue, Stahl, Gregory, Kindy, Mark S, Tomlinson, Stephen
    J Immunol, 2012;189(9):4640-7.  2012
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    Histol. Histopathol., 2010;25(0):1557-71.  2010
  42. Folate Receptor-Targeted Polymeric Micellar Nanocarriers for Delivery of Orlistat as a Repurposed Drug against Triple-Negative Breast Cancer.
    Authors: Paulmurugan R, Bhethanabotla R, Mishra K, Devulapally R, Foygel K, Sekar T, Ananta J, Massoud T, Joy A
    Mol Cancer Ther, 0;15(2):221-31.  0
  43. Experimental mild renal insufficiency mediates early cardiac apoptosis, fibrosis, and diastolic dysfunction: a kidney-heart connection.
    Authors: Martin F, McKie P, Cataliotti A, Sangaralingham S, Korinek J, Huntley B, Oehler E, Harders G, Ichiki T, Mangiafico S, Nath K, Redfield M, Chen H, Burnett J
    Am J Physiol Regul Integr Comp Physiol, 0;302(2):R292-9.  0
  44. Reovirus uses multiple endocytic pathways for cell entry.
    Authors: Schulz W, Haj A, Schiff L
    J Virol, 0;86(23):12665-75.  0
  45. Cryopreservation Method for the Effective Collection of Dental Pulp Stem Cells.
    Authors: Takebe Y, Tatehara S, Fukushima T, Tokuyama-Toda R, Yasuhara R, Mishima K, Satomura K
    Tissue Eng Part C Methods, 0;23(5):251-261.  0
  46. Acceleration of diabetic wound healing using a novel protease-anti-protease combination therapy.
    Authors: Gao M, Nguyen T, Suckow M, Wolter W, Gooyit M, Mobashery S, Chang M
    Proc Natl Acad Sci U S A, 0;112(49):15226-31.  0
  47. Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell abundance in partial nephrectomized mice.
    Authors: Drummond C, Sayed M, Evans K, Shi H, Wang X, Haller S, Liu J, Cooper C, Xie Z, Shapiro J, Tian J
    Am J Physiol Heart Circ Physiol, 0;306(12):H1631-43.  0
  48. Synthetic lethality of combined glutaminase and Hsp90 inhibition in mTORC1-driven tumor cells.
    Authors: Li J, Csibi A, Yang S, Hoffman G, Li C, Zhang E, Yu J, Blenis J
    Proc Natl Acad Sci U S A, 0;112(1):E21-9.  0
  49. ER stress contributes to renal proximal tubule injury by increasing SREBP-2-mediated lipid accumulation and apoptotic cell death.
    Authors: Lhotak S, Sood S, Brimble E, Carlisle R, Colgan S, Mazzetti A, Dickhout J, Ingram A, Austin R
    Am J Physiol Renal Physiol, 0;303(2):F266-78.  0
  50. Uterine natural killer cell partnerships in early mouse decidua basalis.
    Authors: Felker A, Croy B
    J Leukoc Biol, 0;100(4):645-655.  0
  51. IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice.
    Authors: Ferreira T, de Arantes A, do Nascimento C, Olsen P, Trentin P, Rocco P, Hogaboam C, Puri R, Martins M, Silva P
    J Immunol, 0;191(10):5220-9.  0
  52. The endogenous cell-fate factor dachshund restrains prostate epithelial cell migration via repression of cytokine secretion via a cxcl signaling module.
    Authors: Chen K, Wu K, Jiao X, Wang L, Ju X, Wang M, Di Sante G, Xu S, Wang Q, Li K, Sun X, Xu C, Li Z, Casimiro M, Ertel A, Addya S, McCue P, Lisanti M, Wang C, Davis R, Mardon G, Pestell R
    Cancer Res, 0;75(10):1992-2004.  0
  53. Tumor promoter-induced sulfiredoxin is required for mouse skin tumorigenesis.
    Authors: Wu L, Jiang H, Chawsheen H, Mishra M, Young M, Gerard M, Toledano M, Colburn N, Wei Q
    Carcinogenesis, 0;35(5):1177-84.  0
  54. Secreted Frizzled-Related Protein 3 (SFRP3) Is Required for Tumorigenesis of PAX3-FOXO1-Positive Alveolar Rhabdomyosarcoma.
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  55. Vanadium toxicity in mice: possible impairment of lipid metabolism and mucosal epithelial cell necrosis in the small intestine.
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    Toxicol Pathol, 0;41(6):842-56.  0
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  57. Regulation of Sirtuin 3-Mediated Deacetylation of Cyclophilin D Attenuated Cognitive Dysfunction Induced by Sepsis-Associated Encephalopathy in Mice.
    Authors: Sun F, Si Y, Bao H, Xu Y, Pan X, Zeng L, Jing L
    Cell Mol Neurobiol, 0;37(8):1457-1464.  0
  58. mTORC1-Driven Tumor Cells Are Highly Sensitive to Therapeutic Targeting by Antagonists of Oxidative Stress.
    Authors: Li J, Shin S, Sun Y, Yoon S, Li C, Zhang E, Yu J, Zhang J, Blenis J
    Cancer Res, 0;76(16):4816-27.  0
  59. Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.
    Authors: Gornicka A, Fettig J, Eguchi A, Berk M, Thapaliya S, Dixon L, Feldstein A
    Am J Physiol Endocrinol Metab, 0;303(5):E597-606.  0
  60. Differential cell-matrix responses in hypoxia-stimulated aortic versus mitral valves.
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  61. Distinct Immune Responses in Resistant and Susceptible Strains of Mice during Neurovirulent Alphavirus Encephalomyelitis.
    Authors: Kulcsar K, Baxter V, Abraham R, Nelson A, Griffin D
    J Virol, 0;89(16):8280-91.  0
  62. Bacterial chemotaxis modulates host cell apoptosis to establish a T-helper cell, type 17 (Th17)-dominant immune response in Helicobacter pylori infection.
    Authors: Rolig A, Carter J, Ottemann K
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  63. Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development.
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    Mol Cell Biol, 0;36(17):2282-99.  0
  64. Suppression of heat shock protein 27 induces long-term dormancy in human breast cancer.
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  65. Neonatal uterine and vaginal cell proliferation and adenogenesis are independent of estrogen receptor 1 (ESR1) in the mouse.
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    Biol Reprod, 0;92(3):78.  0
  66. Intestinal myofibroblast-specific Tpl2-Cox-2-PGE2 pathway links innate sensing to epithelial homeostasis.
    Authors: Roulis M, Nikolaou C, Kotsaki E, Kaffe E, Karagianni N, Koliaraki V, Salpea K, Ragoussis J, Aidinis V, Martini E, Becker C, Herschman H, Vetrano S, Danese S, Kollias G
    Proc Natl Acad Sci U S A, 0;111(43):E4658-67.  0
  67. TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord.
    Authors: Church J, Kigerl K, Lerch J, Popovich P, McTigue D
    J Neurosci, 0;36(23):6352-64.  0
  68. Targeted Deletion of p53 in Lgr5-Expressing Intestinal Stem Cells Promotes Colon Tumorigenesis in a Preclinical Model of Colitis-Associated Cancer.
    Authors: Davidson L, Callaway E, Kim E, Weeks B, Fan Y, Allred C, Chapkin R
    Cancer Res, 0;75(24):5392-7.  0
  69. Transient increase of fractional anisotropy in reversible vasogenic edema.
    Authors: Kimura-Ohba S, Yang Y, Thompson J, Kimura T, Salayandia V, Cosse M, Yang Y, Sillerud L, Rosenberg G
    J Cereb Blood Flow Metab, 0;36(10):1731-1743.  0

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TdT In Situ Apoptosis Detection Kit - DAB
By Anonymous on 10/11/2016
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