TACS XTT Cell Proliferation/Viability Assay

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4891-025-K
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Citations (11)
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TACS XTT Cell Proliferation/Viability Assay Summary

TACS® XTT Cell Proliferation/Viability Assay

Relative Cell Number Measured with XTT. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densityies. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 27° C and absorbances were read using an ELISA plate reader.

XTT can be used to assay cell proliferation, cell viability, and/or cytotoxicity. The procedure avoids radioactivity and gives reproducible and sensitive results. XTT is added directly to the culture medium and cleavage of the tetrazolium salt to formazan occurs in metabolically active cells. The orange formazan dye generated can be measured by absorbance at 490 nm (or 450 nm) in a microplate reader.

Label: Tetrazolium salt XTT changes from yellow to orange
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: 25 x 96-well microplates


TACS is a registered trademark of Trevigen, Inc.

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Preparation and Storage

Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Citations for TACS XTT Cell Proliferation/Viability Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Intraocular DHODH-inhibitor PP-001 suppresses relapsing experimental uveitis and cytokine production of human lymphocytes, but not of RPE cells
    Authors: M Diedrichs-, S Niesik, CS Priglinger, SR Thurau, F Obermayr, S Sperl, G Wildner
    J Neuroinflammation, 2018;15(1):54.  2018
  2. Stabilization of telomere G-quadruplexes interferes with human herpesvirus 6A chromosomal integration
    Authors: S Gilbert-Gi, A Gravel, S Artusi, SN Richter, N Wallaschek, BB Kaufer, L Flamand
    J. Virol., 2017;0(0):.  2017
  3. Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells.
    Authors: Hagelgans A, Nacke B, Zamaraeva M, Siegert G, Menschikowski M
    Anticancer Res, 2014;34(4):1723-9.  2014
  4. Akt-activated endothelium constitutes the niche for residual disease and resistance to bevacizumab in ovarian cancer.
    Authors: Guerrouahen B, Pasquier J, Kaoud N, Maleki M, Beauchamp M, Yasmeen A, Ghiabi P, Lis R, Vidal F, Saleh A, Gotlieb W, Rafii S, Rafii A
    Mol Cancer Ther, 0;13(12):3123-36.  0
  5. Nucleobindin-2 is a positive modulator of EGF-dependent signals leading to enhancement of cell growth and suppression of adipocyte differentiation.
    Authors: Tagaya Y, Miura A, Okada S, Ohshima K, Mori M
    Endocrinology, 0;153(7):3308-19.  0
  6. A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2alpha.
    Authors: Chen S, Hoffman N, Shanmughapriya S, Bao L, Keefer K, Conrad K, Merali S, Takahashi Y, Abraham T, Hirschler-Laszkiewicz I, Wang J, Zhang X, Song J, Barrero C, Shi Y, Kawasawa Y, Bayerl M, Sun T, Barbour M, Wang H, Madesh M, Cheung J, Miller B
    J Biol Chem, 0;289(52):36284-302.  0
  7. Blockade of CCR5 receptor prevents M2 microglia phenotype in a microglia-glioma paradigm.
    Authors: Laudati E, Curro D, Navarra P, Lisi L
    Neurochem Int, 0;108(0):100-108.  0
  8. Methotraxate-Loaded Hybrid Nanoconstructs Target Vascular Lesions and Inhibit Atherosclerosis Progression in ApoE<sup>-/-</sup> Mice.
    Authors: Stigliano C, Ramirez M, Singh J, Aryal S, Key J, Blanco E, Decuzzi P
    Adv Healthc Mater, 0;6(13):.  0
  9. Depletion of the Human Ion Channel TRPM2 in Neuroblastoma Demonstrates Its Key Role in Cell Survival through Modulation of Mitochondrial Reactive Oxygen Species and Bioenergetics.
    Authors: Bao L, Chen S, Conrad K, Keefer K, Abraham T, Lee J, Wang J, Zhang X, Hirschler-Laszkiewicz I, Wang H, Dovat S, Gans B, Madesh M, Cheung J, Miller B
    J Biol Chem, 0;291(47):24449-24464.  0
  10. Anti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3Kdelta/gamma.
    Authors: Davis R, Moore E, Clavijo P, Friedman J, Cash H, Chen Z, Silvin C, Van Waes C, Allen C
    Cancer Res, 0;77(10):2607-2619.  0
  11. Resetting microbiota by Lactobacillus reuteri inhibits T reg deficiency-induced autoimmunity via adenosine A2A receptors.
    Authors: He B, Hoang T, Wang T, Ferris M, Taylor C, Tian X, Luo M, Tran D, Zhou J, Tatevian N, Luo F, Molina J, Blackburn M, Gomez T, Roos S, Rhoads J, Liu Y
    J Exp Med, 0;214(1):107-123.  0

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