Relative Cell Number Measured with XTT. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densityies. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 27° C and absorbances were read using an ELISA plate reader.
XTT can be used to assay cell proliferation, cell viability, and/or cytotoxicity. The procedure avoids radioactivity and gives reproducible and sensitive results. XTT is added directly to the culture medium and cleavage of the tetrazolium salt to formazan occurs in metabolically active cells. The orange formazan dye generated can be measured by absorbance at 490 nm (or 450 nm) in a microplate reader.
Label: Tetrazolium salt XTT changes from yellow to orange Testing Format: Microplate reader Sample Type: Cultured cells Size: 25 x 96-well microplates
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