TACS XTT Cell Proliferation/Viability Assay

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TACS® XTT Cell Proliferation/Viability Assay

Relative Cell Number Measured with XTT. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densityies. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 27° C and absorbances were read using an ELISA plate reader.

XTT can be used to assay cell proliferation, cell viability, and/or cytotoxicity. The procedure avoids radioactivity and gives reproducible and sensitive results. XTT is added directly to the culture medium and cleavage of the tetrazolium salt to formazan occurs in metabolically active cells. The orange formazan dye generated can be measured by absorbance at 490 nm (or 450 nm) in a microplate reader.

Label: Tetrazolium salt XTT changes from yellow to orange
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: 25 x 96-well microplates

TACS is a registered trademark of Trevigen, Inc.

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Preparation and Storage
  • Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. Intraocular DHODH-inhibitor PP-001 suppresses relapsing experimental uveitis and cytokine production of human lymphocytes, but not of RPE cells
    Authors: M Diedrichs-, S Niesik, CS Priglinger, SR Thurau, F Obermayr, S Sperl, G Wildner
    J Neuroinflammation, 2018;15(1):54.  2018
  2. Stabilization of telomere G-quadruplexes interferes with human herpesvirus 6A chromosomal integration
    Authors: S Gilbert-Gi, A Gravel, S Artusi, SN Richter, N Wallaschek, BB Kaufer, L Flamand
    J. Virol., 2017;0(0):.  2017
  3. Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells.
    Authors: Hagelgans A, Nacke B, Zamaraeva M, Siegert G, Menschikowski M
    Anticancer Res, 2014;34(4):1723-9.  2014


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