hh TACS XTT Cell Proliferation/Viability Assay 4891-025-K: R&D Systems

TACS® XTT Cell Proliferation/Viability Assay

Relative Cell Number Measured with XTT. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densityies. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 27° C and absorbances were read using an ELISA plate reader.

XTT can be used to assay cell proliferation, cell viability, and/or cytotoxicity. The procedure avoids radioactivity and gives reproducible and sensitive results. XTT is added directly to the culture medium and cleavage of the tetrazolium salt to formazan occurs in metabolically active cells. The orange formazan dye generated can be measured by absorbance at 490 nm (or 450 nm) in a microplate reader.

Label: Tetrazolium salt XTT changes from yellow to orange
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: 25 x 96-well microplates


TACS is a registered trademark of Trevigen, Inc.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells.
    Authors: Hagelgans A, Nacke B, Zamaraeva M, Siegert G, Menschikowski M
    Anticancer Res, 2014;34(4):1723-9. 2014
Mycoplasma Detection
Description Application Cat# Citations Images  

MycoProbe Mycoplasma Detection Kit

CUL001B 29
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used TACS XTT Cell Proliferation/Viability Assay?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

R&D Systems Guarantee

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information