TAF15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32461

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TAF15 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAF15. The exact sequence is proprietary.

Reactivity Notes

Negative Xenopus reactivity reported by customer review.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TAF15 Antibody - BSA Free

Western Blot: TAF15 Antibody [NBP1-32461]

Western Blot: TAF15 Antibody [NBP1-32461]

Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: TAF15 Antibody [NBP1-32461]

Immunocytochemistry/ Immunofluorescence: TAF15 Antibody [NBP1-32461]

Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP1-32461] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461] - Paraffin-embedded human breast carcinoma. TAFII68 antibody [N1N3] diluted at 1:250.
Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461]

Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.

Applications for TAF15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAF15

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.

Alternate Names

68kDa, hTAFII68, Npl3, RBP56TAFII68, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2NRBP56/CSMF fusion, TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56), TATA box-binding protein-associated factor 2N (RNA-binding protein 56), TATA-binding protein-associated factor 2N, TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Entrez Gene IDs

8148 (Human)

Gene Symbol

TAF15

UniProt

Q92804 (Human)

Additional TAF15 Products

Product Documents for TAF15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TAF15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TAF15 Antibody - BSA Free

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  • Q: Has anyone ever tried this antibody in Xenopus? If so, what is the reference?

    A:

    This antibody has not yet been tested in Xenopus. There are only unreviewed Xenopus TAF15 sequences on UniProt, so I am not sure how accurate they are. I carried out a sequence comparison, and the percent homology with the human immunogen looks to be about 40-50%, which is quite low, suggesting that the antibody probably won't bind, unfortunately. If you did wish to try this antibody with Xenopus, note that you would be eligible for our Innovator's Rewards program, where Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we would send you a form for you to submit a summary of your research and report your experimental results, after which your award would be given, regardless of whether or not the antibody proved suitable for the species.

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