Talin1 Antibody (8D4)

Novus Biologicals | Catalog # NBP3-23429

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D4
View all Talin1 Primary Antibodies »

Product Specifications

Immunogen

Clone 8D4 was generated from purified chicken gizzard Talin1. The antibody recognizes an epitope within the rod domain of Talin1 from many species, including human, rat, mouse, chicken, and frog. [UniProt# P54939]

Specificity

The antibody detects a 240 kDa protein corresponding to the molecular mass of Talin1 on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Talin1 Antibody (8D4)

Talin1 Antibody (8D4)

Western Blot: Talin1 Antibody (8D4) [NBP3-23429] -

Western Blot: Talin1 Antibody (8D4) [NBP3-23429] - Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin1 (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin1 (Ser-425) (lanes 3 & 4) antibodies.

Applications for Talin1 Antibody (8D4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Talin1

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Alternate Names

KIAA1027, TLN1

Gene Symbol

TLN1

Additional Talin1 Products

Product Documents for Talin1 Antibody (8D4)

Certificate of Analysis

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Product Specific Notices for Talin1 Antibody (8D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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