TAP Tag Antibody (22F12-1E3)

Novus Biologicals | Catalog # NBP2-54891

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Non-species specific

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 22F12-1E3
View all TAP Tag Primary Antibodies »

Product Specifications

Immunogen

KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage

Reactivity Notes

TAG

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TAP Tag Antibody (22F12-1E3)

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891]

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891]

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891] - WB analysis of TAP tag.
Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891]

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891]

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891] - WB analysis of TAP tag.

Applications for TAP Tag Antibody (22F12-1E3)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:1000 - 1:2000
Application Notes
Detects TAP in WB applications. The immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TAP Tag

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

Alternate Names

tandem affinity purification, tandem affinity purification tag, TAP

Additional TAP Tag Products

Product Documents for TAP Tag Antibody (22F12-1E3)

Certificate of Analysis

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Product Specific Notices for TAP Tag Antibody (22F12-1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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