TBCE Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-06359

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TBCE Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human TBCE fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TBCE Antibody - BSA Free

Western Blot: TBCE Antibody [NBP3-06359]

Western Blot: TBCE Antibody [NBP3-06359]

Western Blot: TBCE Antibody [NBP3-06359] - Anti-TBCE rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:55 kDa
Immunohistochemistry-Paraffin: TBCE Antibody [NBP3-06359]

Immunohistochemistry-Paraffin: TBCE Antibody [NBP3-06359]

Immunohistochemistry-Paraffin: TBCE Antibody [NBP3-06359] - Immunochemical staining of human TBCE in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: TBCE Antibody [NBP3-06359]

Immunoprecipitation: TBCE Antibody [NBP3-06359]

Immunoprecipitation: TBCE Antibody [NBP3-06359] - TBCE was immunoprecipitated using:Lane A:0.5 mg Jurkat HeLa Whole Cell Lysate4 uL anti-TBCE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-TBCE rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa

Applications for TBCE Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBCE

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

HRDTubulin-folding cofactor E, KCS1KCS, Kenny-Caffey syndrome, pac2, tubulin folding cofactor E, tubulin-specific chaperone e

Gene Symbol

TBCE

Additional TBCE Products

Product Documents for TBCE Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TBCE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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