TC21/R-Ras2 Antibody (EM-50) - BSA Free

Novus Biologicals | Catalog # NBP2-62216

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # EM-50

Format

BSA Free
Loading...

Product Specifications

Immunogen

Human TC21

Specificity

The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TC21/R-Ras2 Antibody (EM-50) - BSA Free

Immunocytochemistry/ Immunofluorescence: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Immunocytochemistry/ Immunofluorescence: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Immunocytochemistry/Immunofluorescence: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP construct, after fixation and permeabilization. R-Ras2/TC21 was detected using monoclonal antibody EM-50, purified.
Flow Cytometry: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Flow Cytometry: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Flow Cytometry: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2-GFP transfectants using monoclonal antibody EM-50, purified / GAM-APC.
Flow (Intracellular): TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Flow (Intracellular): TC21/R-Ras2 Antibody (EM-50) [NBP2-62216]

Flow (Intracellular): TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2 transfectants using monoclonal antibody EM-50, PE.

Applications for TC21/R-Ras2 Antibody (EM-50) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow (Intracellular)

1-4 ug/ml

Flow Cytometry

1-4 ug/ml

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TC21/R-Ras2

RRAS2 (Related RAS viral oncogene homolog 2) is a member of the Ras superfamily. It is a GTP-binding protein with GTPase activity. It might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins. RRAS2 has high oncogenic potential and is mutated in some human tumors and overexpressed in breast cancer cell lines.

Long Name

Teratocarcinoma Oncogene TC21

Alternate Names

RRAS2

Gene Symbol

RRAS2

Additional TC21/R-Ras2 Products

Product Documents for TC21/R-Ras2 Antibody (EM-50) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TC21/R-Ras2 Antibody (EM-50) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TC21/R-Ras2 Antibody (EM-50) - BSA Free

There are currently no reviews for this product. Be the first to review TC21/R-Ras2 Antibody (EM-50) - BSA Free and earn rewards!

Have you used TC21/R-Ras2 Antibody (EM-50) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...