TcBuster™-M GMP Transposase mRNA

TcBuster™-M GMP Transposase mRNA Summary

Key Benefits

• Suitable for preclinical or ex vivo clinical use
• Delivers large, multicistronic DNA cargo
• Efficient and stable gene delivery for a wide range of genome engineering applications
• Integrates cargo with reduced preference for transcript regions compared to lentivirus
• Combines with other gene editing reagents, such as CRISPR, in one electroporation step
• Reduces time and costs compared to traditional viral vector-based technologies

Why Use TcBuster-M GMP Transposase mRNA and the TcBuster Non-Viral Gene Delivery System?

Gene delivery is a crucial step for a wide variety of genome engineering workflows, which have traditionally utilized viral vectors to achieve expression of exogenous DNA sequences. However, the generation of a virus is time consuming, inconsistent, and has limited cargo capacity. TcBuster-M Transposase mRNA, in combination with a compatible DNA TcBuster transposon (plasmid), form the TcBuster non-viral gene delivery system. The TcBuster non-viral gene delivery system is a solution for stable integration of DNA cargo and addresses many of the limitations of viral vectors. This product requires a TcBuster DNA transposon to be functional. Research-grade mRNA and DNA transposon is available for testing from Bio-Techne. For an equivalent RUO version, please view TcBuster-M Transposase mRNA. For control transposons, check out the CD19CAR-DHFR-eGFP Control for cell therapy applications or Insert On eGFP Control for immortalized cell applications. Please contact us for more information on custom or GMP transposons at techsupport@bio-techne.com.

Versatility of TcBuster-M GMP Transposase mRNA & the TcBuster Non-Viral Gene Delivery System

Gene delivery is a critical step in engineering cells for a wide variety of applications. For T and NK immunotherapies, delivery of CAR or TCR DNA cargo is crucial for the potency of the end drug product. Genome engineering involving iPSCs can require stable delivery of complex DNA cargo. For biologics, this system can faciliate stable gene transfer and high titers for work in CHO and HEK cell lines. Because of the diverse applications of the TcBuster system, independent experimental optimization and/or process development will be required. Please visit the TcBuster Non-Viral Gene Engineering page for more information on the system.

Specifications

Product Datasheets