TCEB2 Antibody (6F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00006923-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6F6

Format

Azide and BSA Free
View all TCEB2 Primary Antibodies »

Product Specifications

Immunogen

TCEB2 (NP_009039, 9 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ

Localization

Nuclear

Specificity

TCEB2 - transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCEB2 Antibody (6F6) - Azide and BSA Free

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01]

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01]

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01] - TCEB2 monoclonal antibody (M01), clone 6F6. Analysis of TCEB2 expression in PC-12.
Immunocytochemistry/ Immunofluorescence: TCEB2 Antibody (6F6) [H00006923-M01]

Immunocytochemistry/ Immunofluorescence: TCEB2 Antibody (6F6) [H00006923-M01]

Immunocytochemistry/Immunofluorescence: TCEB2 Antibody (6F6) [H00006923-M01] - Analysis of monoclonal antibody to TCEB2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: TCEB2 Antibody (6F6) [H00006923-M01]

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01]

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01] - TCEB2 monoclonal antibody (M01), clone 6F6 Analysis of TCEB2 expression in Hela S3 NE.
Sandwich ELISA: TCEB2 Antibody (6F6) [H00006923-M01] - Detection limit for recombinant GST tagged TCEB2 is approximately 0.03ng/ml as a capture antibody.

Applications for TCEB2 Antibody (6F6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00006923-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCEB2

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

18-kD subunit, ELOB, Elongin 18 kDa subunit, elongin-B, RNA polymerase II transcription factor SIII p18 subunit, RNA polymerase II transcription factor SIII subunit B, SIII p18, transcription elongation factor B (SIII), polypeptide 2 (18kD, elongin B), transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B), transcription elongation factor B polypeptide 2

Entrez Gene IDs

6923 (Human)

Gene Symbol

ELOB

OMIM

600787 (Human)

UniProt

NP_009039 (Human)

Additional TCEB2 Products

Product Documents for TCEB2 Antibody (6F6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TCEB2 Antibody (6F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TCEB2 Antibody (6F6) - Azide and BSA Free (1)

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  • TCEB2 Antibody (6F6)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colon tissue
    Species: Rat
    Verified Customer | Posted 08/27/2021
    TCEB2 Antibody
    TCEB2 Antibody (6F6) - Azide and BSA Free H00006923-M01

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Protocols

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FAQs

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