TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25713

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1954 expressed in HEK293

Format

Azide and BSA Free
View all TCF-3/E2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human TCF-3/E2A

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (82%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -

Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -

Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - NCI-H929 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -

Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.
TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -

Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

TCF3 / E2A encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Gene Symbol

TCF3

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review TCF-3/E2A Antibody (HL1954) - Azide and BSA Free and earn rewards!

Have you used TCF-3/E2A Antibody (HL1954) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies