TCF-3/E2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20585

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCF-3/E2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.

Reactivity Notes

Rat (83%)

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TCF-3/E2A Antibody - BSA Free (NBP2-20585) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TCF-3/E2A Antibody - BSA Free

Western Blot: TCF-3/E2A Antibody [NBP2-20585]

Western Blot: TCF-3/E2A Antibody [NBP2-20585]

Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.
TCF-3/E2A Antibody

Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] -

Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.
Blue: Fluoroshield with DAPI.
TCF-3/E2A Antibody

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] -

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TCF-3/E2A Antibody

Western Blot: TCF-3/E2A Antibody [NBP2-20585] -

Western Blot: TCF-3/E2A Antibody [NBP2-20585] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF-3/E2A antibody (NBP2-20585) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
TCF-3/E2A Antibody - BSA Free

Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] -

TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 35 ug Jurkat whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 / E2A antibody (NBP2-20585)
7.5 % SDS-PAGE
The immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (NBP2-20585) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for TCF-3/E2A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Gene Symbol

TCF3

UniProt

P15923

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TCF-3/E2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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