TCF7L2 Antibody (1W10T6)

Novus Biologicals | Catalog # NBP3-15768

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1W10T6 expressed in HEK293
View all TCF7L2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TCF7L2 (Q9NQB0). MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF7L2 Antibody (1W10T6)

Western Blot: TCF7L2 Antibody (1W10T6) [NBP3-15768]

Western Blot: TCF7L2 Antibody (1W10T6) [NBP3-15768]

Western Blot: TCF7L2 Antibody (1W10T6) [NBP3-15768] - Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody (NBP3-15768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: TCF7L2 Antibody (1W10T6) [NBP3-15768]

Immunohistochemistry-Paraffin: TCF7L2 Antibody (1W10T6) [NBP3-15768]

Immunohistochemistry-Paraffin: TCF7L2 Antibody (1W10T6) [NBP3-15768] - Immunohistochemistry of paraffin-embedded rat testis using TCF4/TCF7L2 antibody (NBP3-15768) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
TCF7L2 Antibody (1W10T6)

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] -

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TCF7L2 Antibody (1W10T6)

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] -

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TCF7L2 Antibody (1W10T6)

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] -

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
TCF7L2 Antibody (1W10T6)

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] -

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
TCF7L2 Antibody (1W10T6)

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] -

Immunohistochemistry: TCF7L2 Antibody (1W10T6) [TCF7L2] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using TCF7L2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for TCF7L2 Antibody (1W10T6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCF7L2

TCF7L2, or TCF4, is a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. TCF7L2 has been implicated in glucose homeostasis through the regulation of pro-glucagon gene expression, which encodes glucagon-like peptide 1 (GLP-1). Genetic variants of TCF7L2 have been linked to type 2 diabetes, and it has specifically been shown to have an important role in the regulation of both 946;-cell survival and function (4). Several splicing isoforms for TCF7L2 have been shown to be differentially expressed in tissues during cancer progression (3), and gene targeting studies indicate that TCF7L2 is required to maintain the crypt stem cells of the small intestine (1, 2).

Alternate Names

HMG box transcription factor 4, hTCF-4, T-cell factor 4, T-cell factor-4 variant A, T-cell factor-4 variant B, T-cell factor-4 variant C, T-cell factor-4 variant D, T-cell factor-4 variant E, T-cell factor-4 variant H, T-cell factor-4 variant I, T-cell factor-4 variant J, T-cell factor-4 variant K, T-cell factor-4 variant L, T-cell factor-4 variant M, T-cell factor-4 variant X2, T-cell-specific transcription factor 4, TCF-4T-cell factor-4 variant F, TCF4T-cell factor-4 variant G, transcription factor 7-like 2, transcription factor 7-like 2 (T-cell specific, HMG-box)

Gene Symbol

TCF7L2

Additional TCF7L2 Products

Product Documents for TCF7L2 Antibody (1W10T6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TCF7L2 Antibody (1W10T6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TCF7L2 Antibody (1W10T6)

There are currently no reviews for this product. Be the first to review TCF7L2 Antibody (1W10T6) and earn rewards!

Have you used TCF7L2 Antibody (1W10T6)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies