TCR alpha/beta Antibody (R73) - BSA Free

Novus Biologicals | Catalog # NBP2-12941

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat, Primate

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # R73

Format

BSA Free
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Product Specifications

Immunogen

Rat T blasts and erythrocytes

Reactivity Notes

Non-Human Primates.

Specificity

The mouse monoclonal R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TCR alpha/beta Antibody (R73) - BSA Free

Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.
Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat thymocytes with anti-rat TCR alpha/beta (R73) PE.
TCR alpha/beta Antibody (R73) - BSA Free Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Flow Cytometry: TCR alpha/beta Antibody (R73) - BSA Free [NBP2-12941]

Surface staining pattern of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 ug/ml) GAM APC.

Applications for TCR alpha/beta Antibody (R73) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-12 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 3 using NBP2-12941 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCR alpha/beta

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Alternate Names

C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA

Gene Symbol

TRA

Additional TCR alpha/beta Products

Product Documents for TCR alpha/beta Antibody (R73) - BSA Free

Certificate of Analysis

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Product Specific Notices for TCR alpha/beta Antibody (R73) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCR alpha/beta Antibody (R73) - BSA Free

Customer Reviews for TCR alpha/beta Antibody (R73) - BSA Free (1)

3 out of 5
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  • TCR alpha/beta Antibody (R73)
    Name: Xianying Xing
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 09/07/2018
    I tested on human paraffin embedded tissue and found out that it doesn’t work on human tissue. I used psoriasis lesion tissue and used PH6 and PH9 retrieval buffer but didn’t find any signal. I had a positive control which is a working antibody to make sure the staining system works. The positive control, I used neutrophil elastase(Novus biotechnologies, MAB91671-100) stained on Hidradenitis Suppurativa skin paraffin embedded slides.
    TCR alpha/beta Antibody (R73) - BSA Free NBP2-12941

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