TCR V alpha 12.1 Antibody (6D6.6)
Novus Biologicals | Catalog # NBP2-22491
![Western Blot: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]](https://resources.rndsystems.com/images/products/TCR-V-alpha-12-1-Antibody-6D6-6-Western-Blot-NBP2-22491-img0004.jpg "Western Blot: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]")
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Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6D6.6
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Product Specifications
Immunogen
Human TCR V alpha 12.1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TCR V alpha 12.1 Antibody (6D6.6)
Western Blot: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]
Western Blot: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491] - Analysis of 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates.![Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]](https://resources.rndsystems.com/images/products/TCR-V-alpha-12-1-Antibody-6D6-6-Flow-Cytometry-NBP2-22491-img0003.jpg "Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]")
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491] - Analysis of TCR V alpha 12.1 in Jurkat cells (green) compared to an isotype control (blue).![Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]](https://resources.rndsystems.com/images/products/TCR-V-alpha-12-1-Antibody-6D6-6-Flow-Cytometry-NBP2-22491-img0001.jpg "Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]")
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491] - Analysis of TCR V alpha 12.1 in PBMC cells (green) compared to an isotype control (blue).![Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]](https://resources.rndsystems.com/images/products/TCR-V-alpha-12-1-Antibody-6D6-6-Flow-Cytometry-NBP2-22491-img0002.jpg "Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]")
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491]
Flow Cytometry: TCR V alpha 12.1 Antibody (6D6.6) [NBP2-22491] - Analysis of TCR V alpha 12.1 in PBMC cells (green) compared to an isotype control (blue).Applications for TCR V alpha 12.1 Antibody (6D6.6)
Application
Recommended Usage
Flow Cytometry
1 - 2 ug
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:1 - 1:100
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 0.5% BSA
Preservative
0.1% Sodium Azide
Concentration
0.15 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TCR V alpha 12.1
Alternate Names
T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha cluster, TCR V alpha 12, TCRA, TCRD, TRA
Entrez Gene IDs
6955 (Human)
Gene Symbol
TRA
UniProt
Additional TCR V alpha 12.1 Products
Product Documents for TCR V alpha 12.1 Antibody (6D6.6)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TCR V alpha 12.1 Antibody (6D6.6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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