TDAG8/GPR65 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-21356

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TDAG8/GPR65 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLIRCILEHAVNFEDHSNSGKRTYTMYRI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TDAG8/GPR65 Antibody - BSA Free

Western Blot: TDAG8/GPR65 Antibody [NBP3-21356] -

Western Blot: TDAG8/GPR65 Antibody [NBP3-21356] -

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
TDAG8/GPR65 Antibody - BSA Free

TDAG8/GPR65 Antibody - BSA Free-NBP3-21356

Analysis in human tonsil and skeletal muscle tissues using NBP3-21356 antibody. Corresponding GPR65 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Staining of human skeletal muscle shows low expression as expected.
TDAG8/GPR65 Antibody - BSA Free

TDAG8/GPR65 Antibody - BSA Free-NBP3-21356

Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
TDAG8/GPR65 Antibody - BSA Free

TDAG8/GPR65 Antibody - BSA Free-NBP3-21356

Staining of human small intestinal shows strong membranous positivity in lymphoid cells and glandular cells,
TDAG8/GPR65 Antibody - BSA Free

TDAG8/GPR65 Antibody - BSA Free-NBP3-21356

Staining of human stomach shows strong membranous positivity in glandular cells and lymphoid cells
TDAG8/GPR65 Antibody - BSA Free

TDAG8/GPR65 Antibody - BSA Free-NBP3-21356

Staining of human skeletal muscle shows negative positivity in myocytes.

Applications for TDAG8/GPR65 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TDAG8/GPR65

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease. GPR65 has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. ESTs have been isolated from human B-cell/lung/testis, brain/lung/testis, pancreas/spleen, lung/spleen, leukocyte, embryo, kidney, liver/spleen, thyroid, and vessel libraries.

Long Name

G Protein-coupled Receptor 65

Alternate Names

GPR65, Psychosine Receptor

Gene Symbol

GPR65

Additional TDAG8/GPR65 Products

Product Documents for TDAG8/GPR65 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TDAG8/GPR65 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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