TDP-43/TARDBP Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-29648

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TDP-43/TARDBP Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of human TDP-43. Accession # Q13148. Accession # Q13148

Reactivity Notes

The antibody has been directly tested for reactivity in human and rat.

Specificity

Specific for the ~ 43kDa TDP-43 protein in Western blots of rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for TDP-43/TARDBP Antibody - Azide and BSA Free

Western Blot: TDP-43/TARDBP AntibodyAzide and BSA Free [NBP2-29648]

Western Blot: TDP-43/TARDBP AntibodyAzide and BSA Free [NBP2-29648]

Western Blot: TARDBP Antibody [NBP2-29648] - neonatal rat brain lysate showing specific immunolabeling of the ~ 43 kDa TDP43 protein.

Applications for TDP-43/TARDBP Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:250

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS and 30% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TDP-43/TARDBP

TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Entrez Gene IDs

23435 (Human)

Gene Symbol

TARDBP

UniProt

Q13148

Additional TDP-43/TARDBP Products

Product Documents for TDP-43/TARDBP Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TDP-43/TARDBP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

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Protocols

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