TdT In Situ Apoptosis Detection Kit - Core Reagents
TdT In Situ Apoptosis Detection Kit - Core Reagents Summary
Provides a streptavidin-HRP conjugate without an HRP-substrate or a counterstain.Key Benefits
• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Light microscopy or Fluorescence microscopy options.
Why Use the TdT In Situ Apoptosis Detection Kit - Core Reagents?
TACS 2 TdT Core kit utilize unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher's choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.
Product Specifications
- In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Kit Contents
• Streptavidin-HRP
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for TdT In Situ Apoptosis Detection Kit - Core Reagents
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Low-Molecular-Weight beta-1,3-1,6-Glucan Derived from Aureobasidium pullulans Exhibits Anticancer Activity by Inducing Apoptosis in Colorectal Cancer Cells
Authors: JH Kim, J Seo, H No, T Kuge, T Mori, H Kimoto, JK Kim
Biomedicines, 2023-02-11;11(2):. 2023-02-11
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SARS-CoV-2 recombinant spike protein induces cell apoptosis in rat taste buds
Authors: T Yamamoto, Y Koyama, T Ujita, E Sawada, N Kishimoto, K Seo
Journal of dental sciences, 2022-08-26;0(0):. 2022-08-26
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Combined Caloric Restriction and Exercise Provides Greater Metabolic and Neurocognitive Benefits than either as a Monotherapy in Obesity With or Without Estrogen Deprivation
Authors: W Pratchayas, B Arunsak, K Suparan, S Sriwichaii, T Chunchai, N Chattipako, SC Chattipako
The Journal of nutritional biochemistry, 2022-08-14;0(0):109125. 2022-08-14
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Disulfiram inhibits liver fibrosis in rats by suppressing hepatic stellate cell activation and viability
Authors: XM Yang, Z Wu, X Wang, Y Zhou, L Zhu, D Li, HZ Nie, YH Wang, J Li, X Ma
Oncogene, 2022-07-22;23(1):54. 2022-07-22
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Improved bladder contractility after transplantation of human mesenchymal stem cells overexpressing hepatocyte growth factor into underactive bladder from bladder outlet obstruction models of rats
Authors: JH Kim, HJ Yang, SS Choi, SU Kim, HJ Lee, YS Song
PLoS ONE, 2021-12-22;16(12):e0261402. 2021-12-22
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The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation
Authors: Y Ikeda, A Tagami, M Maekawa, A Nagai
Scientific Reports, 2021-02-24;11(1):4486. 2021-02-24
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Decabromodiphenyl ether (BDE-209) exposure to lactating mice perturbs steroidogenesis and spermatogenesis in adult male offspring
Authors: D Sarkar, SK Singh
Ecotoxicology and environmental safety, 2020-12-29;209(0):111783. 2020-12-29
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Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma
Authors: X Wang, EM Langer, CJ Daniel, M Janghorban, V Wu, XJ Wang, RC Sears
Oncogenesis, 2020-09-07;9(9):79. 2020-09-07
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Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells.
Authors: Rizzo A, De Mare V, Rocchi C, Stolfi C, Colantoni A, Neurath M, MacDonald T, Pallone F, Monteleone G, Fantini M
Carcinogenesis, 2014-01-30;35(7):1536-46. 2014-01-30
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Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells.
Authors: Fan Y, Davidson L, Callaway E, Goldsby J, Chapkin R
Carcinogenesis, 2013-12-11;35(3):606-12. 2013-12-11
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Autophagy in pancreatic acinar cells in caerulein-treated mice: immunolocalization of related proteins and their potential as markers of pancreatitis.
Authors: Zhang L, Zhang J, Shea K, Xu L, Tobin G, Knapton A, Sharron S, Rouse R
Toxicol Pathol, 2013-05-02;42(2):435-57. 2013-05-02
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Under-expression of the opioid growth factor receptor promotes progression of human ovarian cancer.
Authors: Donahue R, McLaughlin P, Zagon I
Exp Biol Med (Maywood), 2012-02-10;237(2):167-77. 2012-02-10
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Age-dependent demise of GNAS-mutated skeletal stem cells and "normalization" of fibrous dysplasia of bone.
Authors: Kuznetsov SA, Cherman N, Riminucci M, Collins MT, Robey PG, Bianco P
J. Bone Miner. Res., 2008-11-01;23(11):1731-40. 2008-11-01
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