TdT In Situ Apoptosis Detection Kit - Core Reagents

In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-CK
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TdT In Situ Apoptosis Detection Kit - Core Reagents Summary

Provides a streptavidin-HRP conjugate without an HRP-substrate or a counterstain.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Light microscopy or Fluorescence microscopy options.

Why Use the TdT In Situ Apoptosis Detection Kit - Core Reagents?

TACS 2 TdT Core kit utilize unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher's choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.

Product Specifications

  • In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Kit Contents

• Streptavidin-HRP
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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