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TdT In Situ Apoptosis Detection Kit - Core Reagents

4810-30-CK 5 Citations Datasheet / COA / SDS
In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-CK
Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Background

TdT In Situ Apoptosis Detection Kit - Core Reagents Summary

Provides a streptavidin-HRP conjugate without an HRP-substrate or a counterstain.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Light microscopy or Fluorescence microscopy options.

Why Use the TdT In Situ Apoptosis Detection Kit - Core Reagents?

TACS 2 TdT Core kit utilize unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher's choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.

Product Specifications

  • In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Kit Contents

• Streptavidin-HRP
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Product Datasheet
COA
SDS

Citations for TdT In Situ Apoptosis Detection Kit - Core Reagents

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Age-dependent demise of GNAS-mutated skeletal stem cells and "normalization" of fibrous dysplasia of bone.
    Authors: Kuznetsov SA, Cherman N, Riminucci M, Collins MT, Robey PG, Bianco P
    J. Bone Miner. Res., 2008;23(11):1731-40.  2008
  2. Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells.
    Authors: Rizzo A, De Mare V, Rocchi C, Stolfi C, Colantoni A, Neurath M, MacDonald T, Pallone F, Monteleone G, Fantini M
    Carcinogenesis, 0;35(7):1536-46.  0
  3. Autophagy in pancreatic acinar cells in caerulein-treated mice: immunolocalization of related proteins and their potential as markers of pancreatitis.
    Authors: Zhang L, Zhang J, Shea K, Xu L, Tobin G, Knapton A, Sharron S, Rouse R
    Toxicol Pathol, 0;42(2):435-57.  0
  4. Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells.
    Authors: Fan Y, Davidson L, Callaway E, Goldsby J, Chapkin R
    Carcinogenesis, 0;35(3):606-12.  0
  5. Under-expression of the opioid growth factor receptor promotes progression of human ovarian cancer.
    Authors: Donahue R, McLaughlin P, Zagon I
    Exp Biol Med (Maywood), 0;237(2):167-77.  0

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