TdT In Situ Apoptosis Detection Kit - Fluorescein

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4812-30-K
Product Details
Citations (44)
FAQs
Reviews (1)

TdT In Situ Apoptosis Detection Kit - Fluorescein Summary

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

• TdT based in situ labeling.
• Fluorescence detection.
• Cation optimization option for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin  non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use TdT In Situ Apoptosis Detection Kit - Fluorescein?

Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer. Biotin labeling is detected using a fluorescein conjugate of streptavidin and visualized by epifluorescence microscopy. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Fluorescence microscopy.

Kit Contents

• Proteinase K
• Streptavidin-FITC
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for TdT In Situ Apoptosis Detection Kit - Fluorescein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Chronic high-fat diet consumption induces an alteration in plasma/brain neurotensin signaling, metabolic disturbance, systemic inflammation/oxidative stress, brain apoptosis, and dendritic spine loss
    Authors: N Saiyasit, T Chunchai, N Apaijai, W Pratchayas, J Sripetchwa, N Chattipako, SC Chattipako
    Neuropeptides, 2020;0(0):102047.  2020
  2. Melatonin suppresses senescence-derived mitochondrial dysfunction in mesenchymal stem cells via the HSPA1L-mitophagy pathway
    Authors: JH Lee, YM Yoon, KH Song, H Noh, SH Lee
    Aging Cell, 2020;0(0):e13111.  2020
  3. Valproic Acid and Neural Apoptosis, Inflammation, and Degeneration 30 Days after Traumatic Brain Injury, Hemorrhagic Shock, and Polytrauma in a Swine Model
    Authors: P Chang, AM Williams, UF Bhatti, BE Biestervel, B Liu, VC Nikolian, IS Dennahy, J Lee, Y Li, HB Alam
    J. Am. Coll. Surg., 2019;0(0):.  2019
  4. L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells
    Authors: X Wang, Y Wang, L Zhang, D Zhang, L Bai, W Kong, Y Huang, C Tang, J Du, H Jin
    Oxid Med Cell Longev, 2019;2019(0):1253289.  2019
  5. Survival of Polyploid hybrid salamander embryos
    Authors: ND Charney, JE Kubel, CT Woodard, BI Carbajal-G, S Avis, JA Blyth, CS Eiseman, J Castorino, JH Malone
    BMC Dev. Biol., 2019;19(1):21.  2019
  6. Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes
    Authors: N Mansouri, GR Willis, A Fernandez-, M Reis, S Nassiri, A Mitsialis, S Kourembana
    JCI Insight, 2019;0(0):.  2019
  7. Non-immunogenic utrophin gene therapy for the treatment of muscular dystrophy animal models
    Authors: Y Song, L Morales, AS Malik, AF Mead, CD Greer, MA Mitchell, MT Petrov, LT Su, ME Choi, ST Rosenblum, X Lu, DJ VanBelzen, RK Krishnanku, FJ Balzer, E Loro, R French, KJ Propert, S Zhou, BW Kozyak, PP Nghiem, TS Khurana, JN Kornegay, HH Stedman
    Nat. Med., 2019;0(0):.  2019
  8. 5-ALA/SFC enhances HO-1 expression through the MAPK/Nrf2 antioxidant pathway and attenuates murine tubular epithelial cell apoptosis
    Authors: C Liu, M Fujino, S Zhu, Y Isaka, H Ito, K Takahashi, M Nakajima, T Tanaka, P Zhu, XK Li
    FEBS Open Bio, 2019;0(0):.  2019
  9. Impaired Regeneration Potential in Urinary Stem Cells Diagnosed from the Patients with Diabetic Nephropathy
    Authors: G Xiong, W Tang, D Zhang, D He, G Wei, A Atala, XJ Liang, AJ Bleyer, ME Bleyer, J Yu, JA Aloi, JX Ma, CM Furdui, Y Zhang
    Theranostics, 2019;9(14):4221-4232.  2019
  10. Apoptosis Induction and Alteration of Cell Adherence in Human Lung Cancer Cells under Simulated Microgravity
    Authors: C Dietz, M Infanger, A Romswinkel, F Strube, A Kraus
    Int J Mol Sci, 2019;20(14):.  2019
  11. Cutting Edge: ATM Influences Germinal Center Integrity
    Authors: L Nicolas, M Cols, R Smolkin, KC Fernandez, WT Yewdell, WF Yen, S Zha, BQ Vuong, J Chaudhuri
    J. Immunol., 2019;0(0):.  2019
  12. Combined dipeptidyl peptidase-4 inhibitor with low-dose testosterone exerts greater efficacy than monotherapy on improving brain function in orchiectomized obese rats
    Authors: P Keawtep, W Pratchayas, A Arinno, N Apaijai, T Chunchai, S Kredphoo, T Jaiwongkum, N Chattipako, SC Chattipako
    Exp. Gerontol., 2019;0(0):.  2019
  13. Expression of CCL2 and its receptor in activation and migration of microglia and monocytes induced by photoreceptor apoptosis
    Authors: C Feng, X Wang, T Liu, M Zhang, G Xu, Y Ni
    Mol. Vis., 2017;23(0):765-777.  2017
  14. Irisin-mediated protective effect on LPS-induced acute lung injury via suppressing inflammation and apoptosis of alveolar epithelial cells
    Authors: L Shao, D Meng, F Yang, H Song, D Tang
    Biochem. Biophys. Res. Commun., 2017;487(2):194-200.  2017
  15. Irisin Inhibits Atherosclerosis by Promoting Endothelial Proliferation Through microRNA126-5p
    J Am Heart Assoc, 2016;5(9):.  2016
  16. C/EBP homologous protein modulates liraglutide-mediated attenuation of non-alcoholic steatohepatitis
    Authors: Khalidur Rahman
    Lab Invest, 2016;0(0):.  2016
  17. Integrated Genomics Identifies miR-32/MCL-1 Pathway as a Critical Driver of Melanomagenesis: Implications for miR-Replacement and Combination Therapy
    PLoS ONE, 2016;11(11):e0165102.  2016
  18. Deletion of ErbB4 accelerates polycystic kidney disease progression in cpk mice.
    Authors: Zeng, Fenghua, Miyazawa, Tomoki, Kloepfer, Lance A, Harris, Raymond
    Kidney Int, 2014;86(3):538-47.  2014
  19. Secreted fibroblast-derived miR-34a induces tubular cell apoptosis in fibrotic kidney.
    Authors: Zhou Y, Xiong M, Niu J, Sun Q, Su W, Zen K, Dai C, Yang J
    J Cell Sci, 2014;127(0):4494-506.  2014
  20. Inhibition of JNK phosphorylation by a novel curcumin analog prevents high glucose-induced inflammation and apoptosis in cardiomyocytes and the development of diabetic cardiomyopathy.
    Authors: Pan Y, Wang Y, Zhao Y, Peng K, Li W, Wang Y, Zhang J, Zhou S, Liu Q, Li X, Cai L, Liang G
    Diabetes, 2014;63(10):3497-511.  2014
  21. An orally available small imidazolium salt ameliorates inflammation and fibrosis in a murine model of cholestasis.
    Authors: Ding Z, Kng Y, Yang H, Ke Z, Zhuo L
    Lab. Invest., 2011;91(5):752-63.  2011
  22. Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
    Authors: Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
    PLoS ONE, 2011;6(12):e29166.  2011
  23. Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage.
    Authors: Arita R, Hata Y, Nakao S, Kita T, Miura M, Kawahara S, Zandi S, Almulki L, Tayyari F, Shimokawa H, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2009;58(1):215-26.  2009
  24. A tumor necrosis factor-alpha-mediated pathway promoting autosomal dominant polycystic kidney disease.
    Authors: Li X, Magenheimer BS, Xia S, Johnson T, Wallace DP, Calvet JP, Li R
    Nat. Med., 2008;14(8):863-8.  2008
  25. PhotoPoint photodynamic therapy promotes stabilization of atherosclerotic plaques and inhibits plaque progression.
    Authors: Waksman R, McEwan PE, Moore TI, Pakala R, Kolodgie FD, Hellinga DG, Seabron RC, Rychnovsky SJ, Vasek J, Scott RW, Virmani R
    J. Am. Coll. Cardiol., 2008;52(12):1024-32.  2008
  26. Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
    Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2008;57(10):2784-93.  2008
  27. Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish.
    Authors: Fimbel SM, Montgomery JE, Burket CT, Hyde DR
    J. Neurosci., 2007;27(7):1712-24.  2007
  28. The role of Bcl-xL in synergistic induction of apoptosis by mapatumumab and oxaliplatin in combination with hyperthermia on human colon cancer.
    Authors: Song X, Kim S, Lee Y
    Mol Cancer Res, 0;10(12):1567-79.  0
  29. Carbon monoxide mediates the anti-apoptotic effects of heme oxygenase-1 in medulloblastoma DAOY cells via K+ channel inhibition.
    Authors: Al-Owais M, Scragg J, Dallas M, Boycott H, Warburton P, Chakrabarty A, Boyle J, Peers C
    J Biol Chem, 0;287(29):24754-64.  0
  30. Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice.
    Authors: Planaguma J, Leypoldt F, Mannara F, Gutierrez-Cuesta J, Martin-Garcia E, Aguilar E, Titulaer M, Petit-Pedrol M, Jain A, Balice-Gordon R, Lakadamyali M, Graus F, Maldonado R, Dalmau J
    Brain, 0;138(0):94-109.  0
  31. Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.
    Authors: McLean W, McLean D, Eatock R, Edge A
    Development, 0;143(23):4381-4393.  0
  32. Homeostatic responses of colonic LGR5+ stem cells following acute in vivo exposure to a genotoxic carcinogen.
    Authors: Kim E, Davidson L, Zoh R, Hensel M, Patil B, Jayaprakasha G, Callaway E, Allred C, Turner N, Weeks B, Chapkin R
    Carcinogenesis, 0;37(2):206-14.  0
  33. DHA suppresses chronic apoptosis in the lung caused by perinatal inflammation.
    Authors: Ali M, Heyob K, Velten M, Tipple T, Rogers L
    Am J Physiol Lung Cell Mol Physiol, 0;309(5):L441-8.  0
  34. Thymosin-beta4 prevents cardiac rupture and improves cardiac function in mice with myocardial infarction.
    Authors: Peng H, Xu J, Yang X, Dai X, Peterson E, Carretero O, Rhaleb N
    Am J Physiol Heart Circ Physiol, 0;307(5):H741-51.  0
  35. Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells.
    Authors: Rizzo A, De Mare V, Rocchi C, Stolfi C, Colantoni A, Neurath M, MacDonald T, Pallone F, Monteleone G, Fantini M
    Carcinogenesis, 0;35(7):1536-46.  0
  36. The PARP inhibitors, veliparib and olaparib, are effective chemopreventive agents for delaying mammary tumor development in BRCA1-deficient mice.
    Authors: To C, Kim E, Royce D, Williams C, Collins R, Risingsong R, Sporn M, Liby K
    Cancer Prev Res (Phila), 0;7(7):698-707.  0
  37. Dichloroacetate Prevents Cisplatin-Induced Nephrotoxicity without Compromising Cisplatin Anticancer Properties.
    Authors: Galgamuwa R, Hardy K, Dahlstrom J, Blackburn A, Wium E, Rooke M, Cappello J, Tummala P, Patel H, Chuah A, Tian L, McMorrow L, Board P, Theodoratos A
    J Am Soc Nephrol, 0;27(11):3331-3344.  0
  38. Altered Phenotype of beta-Cells and Other Pancreatic Cell Lineages in Patients With Diffuse Congenital Hyperinsulinism in Infancy Caused by Mutations in the ATP-Sensitive K-Channel.
    Authors: Salisbury R, Han B, Jennings R, Berry A, Stevens A, Mohamed Z, Sugden S, de Krijger R, Cross S, Johnson P, Newbould M, Cosgrove K, Hanley K, Banerjee I, Dunne M, Hanley N
    Diabetes, 0;64(9):3182-8.  0
  39. Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis.
    Authors: Sansanwal P, Li L, Sarwal M
    J Am Soc Nephrol, 0;26(3):612-25.  0
  40. Cucurbitacin I induces protective autophagy in glioblastoma in vitro and in vivo.
    Authors: Yuan G, Yan S, Xue H, Zhang P, Sun J, Li G
    J Biol Chem, 0;289(15):10607-19.  0
  41. Antitumor effect of paclitaxel is mediated by inhibition of myeloid-derived suppressor cells and chronic inflammation in the spontaneous melanoma model.
    Authors: Sevko A, Michels T, Vrohlings M, Umansky L, Beckhove P, Kato M, Shurin G, Shurin M, Umansky V
    J Immunol, 0;190(5):2464-71.  0
  42. In Vitro and In Vivo Tumor Growth Inhibition by Glutathione Disulfide Liposomes.
    Authors: Sadhu S, Wang S, Dachineni R, Averineni R, Yang Y, Yin H, Bhat G, Guan X
    Cancer Growth Metastasis, 0;10(0):1179064417696.  0
  43. Brain-derived neurotrophic factor deficiency restricts proliferation of oligodendrocyte progenitors following cuprizone-induced demyelination.
    Authors: Tsiperson V, Huang Y, Bagayogo I, Song Y, VonDran M, DiCicco-Bloom E, Dreyfus C
    ASN Neuro, 0;7(1):.  0
  44. Persistent Insulin Resistance in Podocytes Caused by Epigenetic Changes of SHP-1 in Diabetes.
    Authors: Lizotte F, Denhez B, Guay A, Gevry N, Cote A, Geraldes P
    Diabetes, 0;65(12):3705-3717.  0

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TdT In Situ Apoptosis Detection Kit - Fluorescein
By Anonymous on 10/01/2018
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