TdT In Situ Apoptosis Detection Kit - Fluorescein

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
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Product Details
Citations (57)
Reviews (2)

TdT In Situ Apoptosis Detection Kit - Fluorescein Summary

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

• TdT based in situ labeling.
• Fluorescence detection.
• Cation optimization option for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin  non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use TdT In Situ Apoptosis Detection Kit - Fluorescein?

Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer. Biotin labeling is detected using a fluorescein conjugate of streptavidin and visualized by epifluorescence microscopy. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Fluorescence microscopy.

Kit Contents

• Proteinase K
• Streptavidin-FITC
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Cytonin


Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

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Citations for TdT In Situ Apoptosis Detection Kit - Fluorescein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Changes of proapoptotic and antiapoptotic genes affect sensitivity to apoptotic stimuli in impaired contractility due to long term bladder outlet obstruction
    Authors: JH Kim, HJ Yang, SS Choi, HJ Lee, YS Song
    PLoS ONE, 2022;17(12):e0279503.  2022
  2. GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes
    Authors: T Manshouri, I Veletic, P Li, CC Yin, SM Post, S Verstovsek, Z Estrov
    Cell Death & Disease, 2022;13(5):481.  2022
  3. Nuclear focal adhesion kinase induces APC/C activator protein CDH1-mediated cyclin-dependent kinase4/6 degradation and inhibits melanoma proliferation
    Authors: JM Murphy, K Jeong, EY Erin Ahn, ST Steve Lim
    The Journal of Biological Chemistry, 2022;0(0):102013.  2022
  4. Geraniin Protects against Cerebral Ischemia/Reperfusion Injury by Suppressing Oxidative Stress and Neuronal Apoptosis via Regulation of the Nrf2/HO-1 Pathway
    Authors: Y Yang, B He, X Zhang, R Yang, X Xia, L Chen, R Li, Z Shen, P Chen
    Oxidative Medicine and Cellular Longevity, 2022;2022(0):2152746.  2022
  5. Oleanolic Acid-conjugated human serum albumin nanoparticles encapsulating doxorubicin as synergistic combination chemotherapy in oropharyngeal carcinoma and melanoma
    Authors: S Kumbham, M Paul, A Itoo, B Ghosh, S Biswas
    International journal of pharmaceutics, 2022;0(0):121479.  2022
  6. Post-ischemia common carotid artery occlusion worsens memory loss, but not sensorimotor deficits, in long-term survived stroke mice
    Authors: Z Yang, X Li, Z Cao, P Wang, DS Warner, H Sheng
    Brain research bulletin, 2022;183(0):153-161.  2022
  7. Synaptic changes and the response of microglia in a light-induced photoreceptor degeneration model
    Authors: S Xu, P Zhang, M Zhang, X Wang, G Li, G Xu, Y Ni
    Molecular Vision, 2021;27(0):206-220.  2021
  8. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa
    Authors: M Yamanaka-T, Y Mizukami, K Sugawara, K Sunami, Y Teranishi, Y Kira, R Paus, D Tsuruta
    International Journal of Molecular Sciences, 2021;22(5):.  2021
  9. Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis
    Authors: X Liu, Y Chai, G Liu, W Su, Q Guo, X Lv, P Gao, B Yu, G Ferbeyre, X Cao, M Wan
    Nature Communications, 2021;12(1):1832.  2021
  10. Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring
    Authors: W Chen, N Liu, S Shen, W Zhu, J Qiao, S Chang, J Dong, M Bai, L Ma, S Wang, W Jia, X Guo, A Li, J Xi, C Jiang, J Kang
    Cell Reports, 2021;37(5):109912.  2021
  11. SMC4 knockdown inhibits malignant biological behaviors of endometrial cancer cells by regulation of FoxO1 activity
    Authors: Y Yan, C Liu, J Zhang, W Li, X Yin, L Dong, S Pang, X Li
    Archives of biochemistry and biophysics, 2021;712(0):109026.  2021
  12. Defactinib inhibits PYK2 phosphorylation of IRF5 and reduces intestinal inflammation
    Authors: G Ryzhakov, H Almuttaqi, AL Corbin, DL Berthold, T Khoyratty, HL Eames, S Bullers, C Pearson, Z Ai, K Zec, S Bonham, R Fischer, L Jostins-De, SPL Travis, BM Kessler, IA Udalova
    Nature Communications, 2021;12(1):6702.  2021
  13. Redox homeostasis and cell cycle activation mediate beta-cell mass expansion in aged, diabetes-prone mice under metabolic stress conditions: Role of thioredoxin-interacting protein (TXNIP)
    Authors: R Kehm, M Jähnert, S Deubel, T Flore, J König, T Jung, M Stadion, W Jonas, A Schürmann, T Grune, A Höhn
    Redox Biol, 2020;37(0):101748.  2020
  14. Chronic high-fat diet consumption induces an alteration in plasma/brain neurotensin signaling, metabolic disturbance, systemic inflammation/oxidative stress, brain apoptosis, and dendritic spine loss
    Authors: N Saiyasit, T Chunchai, N Apaijai, W Pratchayas, J Sripetchwa, N Chattipako, SC Chattipako
    Neuropeptides, 2020;0(0):102047.  2020
  15. Melatonin suppresses senescence-derived mitochondrial dysfunction in mesenchymal stem cells via the HSPA1L-mitophagy pathway
    Authors: JH Lee, YM Yoon, KH Song, H Noh, SH Lee
    Aging Cell, 2020;0(0):e13111.  2020
  16. Valproic Acid and Neural Apoptosis, Inflammation, and Degeneration 30 Days after Traumatic Brain Injury, Hemorrhagic Shock, and Polytrauma in a Swine Model
    Authors: P Chang, AM Williams, UF Bhatti, BE Biestervel, B Liu, VC Nikolian, IS Dennahy, J Lee, Y Li, HB Alam
    J. Am. Coll. Surg., 2019;0(0):.  2019
  17. L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells
    Authors: X Wang, Y Wang, L Zhang, D Zhang, L Bai, W Kong, Y Huang, C Tang, J Du, H Jin
    Oxid Med Cell Longev, 2019;2019(0):1253289.  2019
  18. Survival of Polyploid hybrid salamander embryos
    Authors: ND Charney, JE Kubel, CT Woodard, BI Carbajal-G, S Avis, JA Blyth, CS Eiseman, J Castorino, JH Malone
    BMC Dev. Biol., 2019;19(1):21.  2019
  19. Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes
    Authors: N Mansouri, GR Willis, A Fernandez-, M Reis, S Nassiri, A Mitsialis, S Kourembana
    JCI Insight, 2019;0(0):.  2019
  20. Non-immunogenic utrophin gene therapy for the treatment of muscular dystrophy animal models
    Authors: Y Song, L Morales, AS Malik, AF Mead, CD Greer, MA Mitchell, MT Petrov, LT Su, ME Choi, ST Rosenblum, X Lu, DJ VanBelzen, RK Krishnanku, FJ Balzer, E Loro, R French, KJ Propert, S Zhou, BW Kozyak, PP Nghiem, TS Khurana, JN Kornegay, HH Stedman
    Nat. Med., 2019;0(0):.  2019
  21. 5-ALA/SFC enhances HO-1 expression through the MAPK/Nrf2 antioxidant pathway and attenuates murine tubular epithelial cell apoptosis
    Authors: C Liu, M Fujino, S Zhu, Y Isaka, H Ito, K Takahashi, M Nakajima, T Tanaka, P Zhu, XK Li
    FEBS Open Bio, 2019;0(0):.  2019
  22. Impaired Regeneration Potential in Urinary Stem Cells Diagnosed from the Patients with Diabetic Nephropathy
    Authors: G Xiong, W Tang, D Zhang, D He, G Wei, A Atala, XJ Liang, AJ Bleyer, ME Bleyer, J Yu, JA Aloi, JX Ma, CM Furdui, Y Zhang
    Theranostics, 2019;9(14):4221-4232.  2019
  23. Apoptosis Induction and Alteration of Cell Adherence in Human Lung Cancer Cells under Simulated Microgravity
    Authors: C Dietz, M Infanger, A Romswinkel, F Strube, A Kraus
    Int J Mol Sci, 2019;20(14):.  2019
  24. Cutting Edge: ATM Influences Germinal Center Integrity
    Authors: L Nicolas, M Cols, R Smolkin, KC Fernandez, WT Yewdell, WF Yen, S Zha, BQ Vuong, J Chaudhuri
    J. Immunol., 2019;0(0):.  2019
  25. Combined dipeptidyl peptidase-4 inhibitor with low-dose testosterone exerts greater efficacy than monotherapy on improving brain function in orchiectomized obese rats
    Authors: P Keawtep, W Pratchayas, A Arinno, N Apaijai, T Chunchai, S Kredphoo, T Jaiwongkum, N Chattipako, SC Chattipako
    Exp. Gerontol., 2019;0(0):.  2019
  26. Expression of CCL2 and its receptor in activation and migration of microglia and monocytes induced by photoreceptor apoptosis
    Authors: C Feng, X Wang, T Liu, M Zhang, G Xu, Y Ni
    Mol. Vis., 2017;23(0):765-777.  2017
  27. Irisin-mediated protective effect on LPS-induced acute lung injury via suppressing inflammation and apoptosis of alveolar epithelial cells
    Authors: L Shao, D Meng, F Yang, H Song, D Tang
    Biochem. Biophys. Res. Commun., 2017;487(2):194-200.  2017
  28. Integrated Genomics Identifies miR-32/MCL-1 Pathway as a Critical Driver of Melanomagenesis: Implications for miR-Replacement and Combination Therapy
    PLoS ONE, 2016;11(11):e0165102.  2016
  29. C/EBP homologous protein modulates liraglutide-mediated attenuation of non-alcoholic steatohepatitis
    Authors: Khalidur Rahman
    Lab Invest, 2016;0(0):.  2016
  30. Irisin Inhibits Atherosclerosis by Promoting Endothelial Proliferation Through microRNA126-5p
    J Am Heart Assoc, 2016;5(9):.  2016
  31. Inhibition of JNK phosphorylation by a novel curcumin analog prevents high glucose-induced inflammation and apoptosis in cardiomyocytes and the development of diabetic cardiomyopathy.
    Authors: Pan Y, Wang Y, Zhao Y, Peng K, Li W, Wang Y, Zhang J, Zhou S, Liu Q, Li X, Cai L, Liang G
    Diabetes, 2014;63(10):3497-511.  2014
  32. Deletion of ErbB4 accelerates polycystic kidney disease progression in cpk mice.
    Authors: Zeng, Fenghua, Miyazawa, Tomoki, Kloepfer, Lance A, Harris, Raymond
    Kidney Int, 2014;86(3):538-47.  2014
  33. Secreted fibroblast-derived miR-34a induces tubular cell apoptosis in fibrotic kidney.
    Authors: Zhou Y, Xiong M, Niu J, Sun Q, Su W, Zen K, Dai C, Yang J
    J Cell Sci, 2014;127(0):4494-506.  2014
  34. An orally available small imidazolium salt ameliorates inflammation and fibrosis in a murine model of cholestasis.
    Authors: Ding Z, Kng Y, Yang H, Ke Z, Zhuo L
    Lab. Invest., 2011;91(5):752-63.  2011
  35. Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
    Authors: Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
    PLoS ONE, 2011;6(12):e29166.  2011
  36. Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage.
    Authors: Arita R, Hata Y, Nakao S, Kita T, Miura M, Kawahara S, Zandi S, Almulki L, Tayyari F, Shimokawa H, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2009;58(1):215-26.  2009
  37. A tumor necrosis factor-alpha-mediated pathway promoting autosomal dominant polycystic kidney disease.
    Authors: Li X, Magenheimer BS, Xia S, Johnson T, Wallace DP, Calvet JP, Li R
    Nat. Med., 2008;14(8):863-8.  2008
  38. Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
    Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2008;57(10):2784-93.  2008
  39. PhotoPoint photodynamic therapy promotes stabilization of atherosclerotic plaques and inhibits plaque progression.
    Authors: Waksman R, McEwan PE, Moore TI, Pakala R, Kolodgie FD, Hellinga DG, Seabron RC, Rychnovsky SJ, Vasek J, Scott RW, Virmani R
    J. Am. Coll. Cardiol., 2008;52(12):1024-32.  2008
  40. Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish.
    Authors: Fimbel SM, Montgomery JE, Burket CT, Hyde DR
    J. Neurosci., 2007;27(7):1712-24.  2007
  41. Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice.
    Authors: Planaguma J, Leypoldt F, Mannara F, Gutierrez-Cuesta J, Martin-Garcia E, Aguilar E, Titulaer M, Petit-Pedrol M, Jain A, Balice-Gordon R, Lakadamyali M, Graus F, Maldonado R, Dalmau J
    Brain, 0;138(0):94-109.  0
  42. The role of Bcl-xL in synergistic induction of apoptosis by mapatumumab and oxaliplatin in combination with hyperthermia on human colon cancer.
    Authors: Song X, Kim S, Lee Y
    Mol Cancer Res, 0;10(12):1567-79.  0
  43. Carbon monoxide mediates the anti-apoptotic effects of heme oxygenase-1 in medulloblastoma DAOY cells via K+ channel inhibition.
    Authors: Al-Owais M, Scragg J, Dallas M, Boycott H, Warburton P, Chakrabarty A, Boyle J, Peers C
    J Biol Chem, 0;287(29):24754-64.  0
  44. Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.
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    Development, 0;143(23):4381-4393.  0
  45. Thymosin-beta4 prevents cardiac rupture and improves cardiac function in mice with myocardial infarction.
    Authors: Peng H, Xu J, Yang X, Dai X, Peterson E, Carretero O, Rhaleb N
    Am J Physiol Heart Circ Physiol, 0;307(5):H741-51.  0
  46. In Vitro and In Vivo Tumor Growth Inhibition by Glutathione Disulfide Liposomes.
    Authors: Sadhu S, Wang S, Dachineni R, Averineni R, Yang Y, Yin H, Bhat G, Guan X
    Cancer Growth Metastasis, 0;10(0):1179064417696.  0
  47. Homeostatic responses of colonic LGR5+ stem cells following acute in vivo exposure to a genotoxic carcinogen.
    Authors: Kim E, Davidson L, Zoh R, Hensel M, Patil B, Jayaprakasha G, Callaway E, Allred C, Turner N, Weeks B, Chapkin R
    Carcinogenesis, 0;37(2):206-14.  0
  48. Brain-derived neurotrophic factor deficiency restricts proliferation of oligodendrocyte progenitors following cuprizone-induced demyelination.
    Authors: Tsiperson V, Huang Y, Bagayogo I, Song Y, VonDran M, DiCicco-Bloom E, Dreyfus C
    ASN Neuro, 0;7(1):.  0
  49. Antitumor effect of paclitaxel is mediated by inhibition of myeloid-derived suppressor cells and chronic inflammation in the spontaneous melanoma model.
    Authors: Sevko A, Michels T, Vrohlings M, Umansky L, Beckhove P, Kato M, Shurin G, Shurin M, Umansky V
    J Immunol, 0;190(5):2464-71.  0
  50. Cucurbitacin I induces protective autophagy in glioblastoma in vitro and in vivo.
    Authors: Yuan G, Yan S, Xue H, Zhang P, Sun J, Li G
    J Biol Chem, 0;289(15):10607-19.  0
  51. Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis.
    Authors: Sansanwal P, Li L, Sarwal M
    J Am Soc Nephrol, 0;26(3):612-25.  0
  52. Persistent Insulin Resistance in Podocytes Caused by Epigenetic Changes of SHP-1 in Diabetes.
    Authors: Lizotte F, Denhez B, Guay A, Gevry N, Cote A, Geraldes P
    Diabetes, 0;65(12):3705-3717.  0
  53. Dichloroacetate Prevents Cisplatin-Induced Nephrotoxicity without Compromising Cisplatin Anticancer Properties.
    Authors: Galgamuwa R, Hardy K, Dahlstrom J, Blackburn A, Wium E, Rooke M, Cappello J, Tummala P, Patel H, Chuah A, Tian L, McMorrow L, Board P, Theodoratos A
    J Am Soc Nephrol, 0;27(11):3331-3344.  0
  54. The PARP inhibitors, veliparib and olaparib, are effective chemopreventive agents for delaying mammary tumor development in BRCA1-deficient mice.
    Authors: To C, Kim E, Royce D, Williams C, Collins R, Risingsong R, Sporn M, Liby K
    Cancer Prev Res (Phila), 0;7(7):698-707.  0
  55. Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells.
    Authors: Rizzo A, De Mare V, Rocchi C, Stolfi C, Colantoni A, Neurath M, MacDonald T, Pallone F, Monteleone G, Fantini M
    Carcinogenesis, 0;35(7):1536-46.  0
  56. Altered Phenotype of beta-Cells and Other Pancreatic Cell Lineages in Patients With Diffuse Congenital Hyperinsulinism in Infancy Caused by Mutations in the ATP-Sensitive K-Channel.
    Authors: Salisbury R, Han B, Jennings R, Berry A, Stevens A, Mohamed Z, Sugden S, de Krijger R, Cross S, Johnson P, Newbould M, Cosgrove K, Hanley K, Banerjee I, Dunne M, Hanley N
    Diabetes, 0;64(9):3182-8.  0
  57. DHA suppresses chronic apoptosis in the lung caused by perinatal inflammation.
    Authors: Ali M, Heyob K, Velten M, Tipple T, Rogers L
    Am J Physiol Lung Cell Mol Physiol, 0;309(5):L441-8.  0


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TdT In Situ Apoptosis Detection Kit - Fluorescein
By Anonymous on 03/04/2021

Best TUNEL kit for staining.

TdT In Situ Apoptosis Detection Kit - Fluorescein
By Anonymous on 10/01/2018