TdT In Situ Apoptosis Detection Kit - Fluorescein
TdT In Situ Apoptosis Detection Kit - Fluorescein Summary
• TdT based in situ labeling.
• Fluorescence detection.
• Cation optimization option for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.
Why Use TdT In Situ Apoptosis Detection Kit - Fluorescein?
Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer. Biotin labeling is detected using a fluorescein conjugate of streptavidin and visualized by epifluorescence microscopy. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.
- In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
- Fluorescence microscopy.
• Proteinase K
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
For research use only. Not for diagnositic use.
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