TdT In Situ Apoptosis Detection Kit - Fluorescein

R&D Systems | Catalog # 4812-30-K

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
R&D Systems
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Key Product Details

Features

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

  • TdT based in situ labeling.
  • Fluorescence detection.
  • Cation optimization option for high signal and low background.
  • Includes both Proteinase K and exclusive Cytonin  non-enzymatic permeabilization reagent.
  • Includes TACS-Nuclease control reagents.

Species

Multi-Species

Product Summary for TdT In Situ Apoptosis Detection Kit - Fluorescein

Why Use TdT In Situ Apoptosis Detection Kit - Fluorescein?
Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer. Biotin labeling is detected using a fluorescein conjugate of streptavidin and visualized by epifluorescence microscopy. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.
Product Specifications
  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Fluorescence microscopy.

Kit Contents for TdT In Situ Apoptosis Detection Kit - Fluorescein

  • Proteinase K
  • Streptavidin-FITC
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • TACS 2 TdT Labeling Buffer
  • TACS 2 TdT Stop Buffer
  • TACS 2 TdT dNTP
  • TdT Enzyme
  • 50x Cobalt Cation
  • 50x Magnesium Cation
  • 50x Manganese Cation
  • Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for TdT In Situ Apoptosis Detection Kit - Fluorescein

Certificate of Analysis

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Citations for TdT In Situ Apoptosis Detection Kit - Fluorescein

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  • TdT In Situ Apoptosis Detection Kit - Fluorescein
    Name: Anonymous
    Verified Customer | Posted 03/04/2021
    Best TUNEL kit for staining.
    TdT In Situ Apoptosis Detection Kit - Fluorescein 4812-30-K
  • TdT In Situ Apoptosis Detection Kit - Fluorescein
    Name: Anonymous
    Verified Customer | Posted 10/01/2018
    TdT In Situ Apoptosis Detection Kit - Fluorescein 4812-30-K

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