TdT In Situ Apoptosis Detection Kit - Fluorescein

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4812-30-K
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TdT In Situ Apoptosis Detection Kit - Fluorescein Summary

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

• TdT based in situ labeling.
• Fluorescence detection.
• Cation optimization option for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin  non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use TdT In Situ Apoptosis Detection Kit - Fluorescein?

Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer. Biotin labeling is detected using a fluorescein conjugate of streptavidin and visualized by epifluorescence microscopy. This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Fluorescence microscopy.

Kit Contents

• Proteinase K
• Streptavidin-FITC
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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