TdT In Situ Apoptosis Detection Kit - TACS Blue Label

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4811-30-K
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TdT In Situ Apoptosis Detection Kit - TACS Blue Label Summary

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Dark blue labeling with red counterstain.

Why Use TdT In Situ Apoptosis Detection Kit - TACS Blue Label?

The TACS 2 TdT Blue Label Kit utilizes unique cation optimization system to enhance in situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrate TACS Blue Label.  Samples are counterstained with Nuclear Fast Red offering a nice contrast when visualized by light microscopy.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• Nuclear Fast Red
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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