TdT In Situ Apoptosis Detection Kit - TACS Blue Label
R&D Systems | Catalog # 4811-30-K
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
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Key Product Details
Features
Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Key Benefits
- TdT based in situ labeling.
- Cation optimization for high signal and low background.
- Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
- Includes TACS-Nuclease control reagents.
- Dark blue labeling with red counterstain.
Species
Multi-Species
Product Summary for TdT In Situ Apoptosis Detection Kit - TACS Blue Label
Why Use TdT In Situ Apoptosis Detection Kit - TACS Blue Label?
The TACS 2 TdT Blue Label Kit utilizes unique cation optimization system to enhance in situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrate TACS Blue Label. Samples are counterstained with Nuclear Fast Red offering a nice contrast when visualized by light microscopy.
Product Specifications
- In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
- Light microscopy.
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Kit Contents for TdT In Situ Apoptosis Detection Kit - TACS Blue Label
- Proteinase K
- Streptavidin-HRP
- TACS-Blue Label
- Blue Strep-HRP Diluent
- TACS-Nuclease
- TACS-Nuclease Buffer
- Nuclear Fast Red
- TACS 2 TdT dNTP
- TdT Enzyme
- 50x Cobalt Cation
- 50x Magnesium Cation
- 50x Manganese Cation
- TACS 2 TdT Labeling Buffer
- TACS 2 TdT Stop Buffer
- Cytonin
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for TdT In Situ Apoptosis Detection Kit - TACS Blue Label
Certificate of Analysis
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Citations for TdT In Situ Apoptosis Detection Kit - TACS Blue Label
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