TdT In Situ Apoptosis Detection Kit - TACS Blue Label

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
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Product Details
Citations (35)

TdT In Situ Apoptosis Detection Kit - TACS Blue Label Summary

Utilizes unique cation optimization system to enhance in situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Dark blue labeling with red counterstain.

Why Use TdT In Situ Apoptosis Detection Kit - TACS Blue Label?

The TACS 2 TdT Blue Label Kit utilizes unique cation optimization system to enhance in situ detection of apoptosis (by TUNEL) in fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrate TACS Blue Label.  Samples are counterstained with Nuclear Fast Red offering a nice contrast when visualized by light microscopy.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• Nuclear Fast Red
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin


Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

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Citations for TdT In Situ Apoptosis Detection Kit - TACS Blue Label

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells.
    Authors: Dassah M, Almeida D, Hahn R, Bonaldo P, Worgall S, Hajjar K
    J Cell Sci, 2104;127(0):828-44.  2104
  2. MG53 Protects against Sepsis-Induced Myocardial Dysfunction by Upregulating Peroxisome Proliferator-Activated Receptor-&alpha
    Authors: X Han, D Chen, N Liufu, F Ji, Q Zeng, W Yao, M Cao
    Oxid Med Cell Longev, 2020;2020(0):7413693.  2020
  3. Genetic Deletion of Vasohibin-2 Exacerbates Ischemia-Reperfusion-Induced Acute Kidney Injury
    Authors: H Miyake, K Tanabe, S Tanimura, Y Nakashima, T Morioka, K Masuda, H Sugiyama, Y Sato, J Wada
    Int J Mol Sci, 2020;21(12):.  2020
  4. The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation -induced mitochondrial and renal injury
    Authors: S Shrum, J Tobacyk, S Lo, N Parajuli, LA MacMillan-
    Arch. Biochem. Biophys., 2020;688(0):108410.  2020
  5. The Brain Penetrating and Dual TORC1/TORC2 Inhibitor, RES529, Elicits Anti-Glioma Activity and Enhances the Therapeutic Effects of Anti-Angiogenetic Compounds in Preclinical Murine Models
    Authors: GL Gravina, A Mancini, A Colapietro, S Delle Mona, R Sferra, S Pompili, F Vitale, S Martellucc, F Marampon, V Mattei, L Biordi, D Sherris, C Festuccia
    Cancers (Basel), 2019;11(10):.  2019
  6. Primary tumor-induced immunity eradicates disseminated tumor cells in syngeneic mouse model
    Authors: R Piranliogl, E Lee, M Ouzounova, RJ Bollag, AH Vinyard, AS Arbab, D Marasco, M Guzel, JK Cowell, M Thangaraju, A Chadli, KA Hassan, MS Wicha, E Celis, H Korkaya
    Nat Commun, 2019;10(1):1430.  2019
  7. UniPR1331, a small molecule targeting Eph/ephrin interaction, prolongs survival in glioblastoma and potentiates the effect of antiangiogenic therapy in mice
    Authors: C Festuccia, GL Gravina, C Giorgio, A Mancini, C Pellegrini, A Colapietro, S Delle Mona, MG Maturo, R Sferra, P Chiodelli, M Rusnati, A Cantoni, R Castelli, F Vacondio, A Lodola, M Tognolini
    Oncotarget, 2018;9(36):24347-24363.  2018
  8. Overcoming sorafenib evasion in hepatocellular carcinoma using CXCR4-targeted nanoparticles to co-deliver MEK-inhibitors
    Authors: Y Chen, YC Liu, YC Sung, RR Ramjiawan, TT Lin, CC Chang, KS Jeng, CF Chang, CH Liu, DY Gao, FF Hsu, AM Duyverman, S Kitahara, P Huang, S Dima, I Popescu, KT Flaherty, AX Zhu, N Bardeesy, RK Jain, CH Benes, DG Duda
    Sci Rep, 2017;7(0):44123.  2017
  9. Osteopontin Impacts West Nile virus Pathogenesis and Resistance by Regulating Inflammasome Components and Cell Death in the Central Nervous System at Early Time Points
    Authors: N Bortell, C Flynn, B Conti, HS Fox, MCG Marcondes
    Mediators Inflamm., 2017;2017(0):7582437.  2017
  10. TNF-? Deficiency Prevents Renal Inflammation and Oxidative Stress in Obese Mice
    Authors: H Wang, J Li, Z Gai, GA Kullak-Ubl, Z Liu
    Kidney Blood Press. Res., 2017;42(3):416-427.  2017
  11. Amine oxidase activity regulates the development of pulmonary fibrosis
    Authors: F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi
    FASEB J, 2017;0(0):.  2017
  12. UCH-L1 is induced in germinal center B cells and identifies patients with aggressive germinal center diffuse large B-cell lymphoma
    Authors: T Bedekovics, S Hussain, AL Feldman, PJ Galardy
    Blood, 2016;127(12):1564-74.  2016
  13. Exosome-mediated delivery of the intrinsic C-terminus domain of PTEN protects it from proteasomal degradation and ablates tumorigenesis.
    Authors: Ahmed, Syed Fer, Das, Nilanjan, Sarkar, Moumita, Chatterjee, Uttara, Chatterjee, Sandip, Ghosh, Mrinal K
    Mol Ther, 2015;23(2):255-69.  2015
  14. Histone deacetylase-mediated silencing of AMWAP expression contributes to cisplatin nephrotoxicity.
    Authors: Ranganathan P, Hamad R, Mohamed R, Jayakumar C, Muthusamy T, Ramesh G
    Kidney Int, 2015;0(0):.  2015
  15. Clever-1/stabilin-1 controls cancer growth and metastasis.
    Authors: Karikoski M, Marttila-Ichihara F, Elima K, Rantakari P, Hollmen M, Kelkka T, Gerke H, Huovinen V, Irjala H, Holmdahl R, Salmi M, Jalkanen S
    Clin Cancer Res, 2014;20(24):6452-64.  2014
  16. UNC5B receptor deletion exacerbates DSS-induced colitis in mice by increasing epithelial cell apoptosis.
    Authors: Ranganathan P, Jayakumar C, Li D, Ramesh G
    J Cell Mol Med, 2014;18(7):1290-9.  2014
  17. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
    Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
    Cell Signal, 2013;25(4):717-28.  2013
  18. The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling.
    Authors: Gui T, Sun Y, Gai Z, Shimokado A, Muragaki Y, Zhou G
    Dev Biol, 2013;377(2):415-27.  2013
  19. VDR hypermethylation and HIV-induced T cell loss.
    Authors: Chandel, Nirupama, Husain, Mohammad, Goel, Hersh, Salhan, Divya, Lan, Xiqian, Malhotra, Ashwani, McGowan, Joseph, Singhal, Pravin C
    J Leukoc Biol, 2013;93(4):623-31.  2013
  20. Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
    Authors: Tadagavadi RK, Wang W
    J. Immunol., 2010;185(0):3750-8.  2010
  21. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
    Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
    Am. J. Pathol., 2009;175(3):1010-8.  2009
  22. Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway.
    Authors: Chamoux E, Houde N, L'Eriger K, Roux S
    J. Cell. Physiol., 2008;216(2):536-42.  2008
  23. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008;314(11):2176-86.  2008
  24. Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis.
    Authors: Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL
    Biochem. Biophys. Res. Commun., 2005;333(1):42-50.  2005
  25. Galacto-oligosaccharides attenuate renal injury with microbiota modification.
    Authors: Furuse S, Ohse T, Jo-Watanabe A, Shigehisa A, Kawakami K, Matsuki T, Chonan O, Nangaku M
    Physiol Rep, 0;2(7):.  0
  26. Epicatechin limits renal injury by mitochondrial protection in cisplatin nephropathy.
    Authors: Tanabe K, Tamura Y, Lanaspa M, Miyazaki M, Suzuki N, Sato W, Maeshima Y, Schreiner G, Villarreal F, Johnson R, Nakagawa T
    Am J Physiol Renal Physiol, 0;303(9):F1264-74.  0
  27. Transcription factor ATF4 directs basal and stress-induced gene expression in the unfolded protein response and cholesterol metabolism in the liver.
    Authors: Fusakio M, Willy J, Wang Y, Mirek E, Al Baghdadi R, Adams C, Anthony T, Wek R
    Mol Biol Cell, 0;27(9):1536-51.  0
  28. EDD enhances cell survival and cisplatin resistance and is a therapeutic target for epithelial ovarian cancer.
    Authors: Bradley A, Zheng H, Ziebarth A, Sakati W, Branham-O'Connor M, Blumer J, Liu Y, Kistner-Griffin E, Rodriguez-Aguayo C, Lopez-Berestein G, Sood A, Landen C, Eblen S
    Carcinogenesis, 0;35(5):1100-9.  0
  29. MYC-driven tumorigenesis is inhibited by WRN syndrome gene deficiency.
    Authors: Moser R, Toyoshima M, Robinson K, Gurley K, Howie H, Davison J, Morgan M, Kemp C, Grandori C
    Mol Cancer Res, 0;10(4):535-45.  0
  30. Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats.
    Authors: Guo Y, Li M, Zhusheng L, Yamada T, Sasaki M, Hasegawa T, Hongo H, Tabata C, Suzuki R, Oda K, Yamamoto T, Kawanami M, Amizuka N
    J Electron Microsc (Tokyo), 0;61(5):309-20.  0
  31. Histological Evidence of Increased Osteoclast Cell Number and Asymmetric Bone Resorption Activity in the Tibiae of Interleukin-6-Deficient Mice.
    Authors: Liu H, Feng W, m, Cui J, Lv S, Hasegawa T, Sun B, Li J, Oda K, Amizuka N, Li M
    J Histochem Cytochem, 0;62(8):556-64.  0
  32. General control nonderepressible 2 deletion predisposes to asparaginase-associated pancreatitis in mice.
    Authors: Phillipson-Weiner L, Mirek E, Wang Y, McAuliffe W, Wek R, Anthony T
    Am J Physiol Gastrointest Liver Physiol, 0;310(11):G1061-70.  0
  33. The eukaryotic initiation factor 2 kinase GCN2 protects against hepatotoxicity during asparaginase treatment.
    Authors: Wilson G, Bunpo P, Cundiff J, Wek R, Anthony T
    Am J Physiol Endocrinol Metab, 0;305(9):E1124-33.  0
  34. Curcumin and resveratrol in combination modulates benzo(a)pyrene-induced genotoxicity during lung carcinogenesis.
    Authors: Malhotra A, Nair P, Dhawan D
    Hum Exp Toxicol, 0;31(12):1199-206.  0
  35. Long-Term Administration of High-Fat Diet Corrects Abnormal Bone Remodeling in the Tibiae of Interleukin-6-Deficient Mice.
    Authors: Feng W, Liu B, Liu D, Hasegawa T, Wang W, Han X, Cui J, m, Oda K, Amizuka N, Li M
    J Histochem Cytochem, 0;64(1):42-53.  0


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