TEAD1 Antibody (13112) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43581

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13112

Format

Azide and BSA Free
View all TEAD1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEAD1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TEAD1 Antibody (13112) - Azide and BSA Free

Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581]

Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581]

Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581] - Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 ug of TEF1 antibody [13112]. Western blot analysis was performed using TEF1 antibody [13112] EasyBlot anti-Mouse IgG was used as a secondary reagent.
TEAD1 Antibody (13112)

Western Blot: TEAD1 Antibody (13112) [NBP2-43581] -

Western Blot: TEAD1 Antibody (13112) [NBP2-43581] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD1 antibody [GT13112] (NBP2-43581) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
TEAD1 Antibody (13112) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TEAD1 Antibody (13112) [NBP2-43581] -

TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TEF1 protein stained by TEF1 antibody [GT13112] (NBP2-43581) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

Applications for TEAD1 Antibody (13112) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TEAD1

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

Long Name

Transcriptional enhancer factor TEF-1

Alternate Names

AA, NTEF-1, protein GT-IIC, REF1, TCF13, TCF-13, TEA domain family member 1 (SV40 transcriptional enhancer factor), TEAD1, TEAD-1, TEF-1, transcription factor 13, transcriptional enhancer factor 1, transcriptional enhancer factor TEF-1

Gene Symbol

TEAD1

Additional TEAD1 Products

Product Documents for TEAD1 Antibody (13112) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TEAD1 Antibody (13112) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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