Testin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85072

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Testin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YCDSEKPRCAGCDELIFSNEYTQAENQNWHLKHFCCFDCDSILAGEIYVMVNDKPVCKPCYVKNHAVVCQGCHNAIDPEVQRVTYNNFSWHASTECFLCSCCSKCLIGQKFMPVEGMVFCSVECKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Testin Antibody - BSA Free

Western Blot: Testin Antibody [NBP1-85072]

Western Blot: Testin Antibody [NBP1-85072]

Western Blot: Testin Antibody [NBP1-85072] - Analysis using Anti-TES antibody NBP1-85072 (A) shows similar pattern to independent antibody NBP1-87987 (B).
Immunocytochemistry/ Immunofluorescence: Testin Antibody [NBP1-85072]

Immunocytochemistry/ Immunofluorescence: Testin Antibody [NBP1-85072]

Immunocytochemistry/Immunofluorescence: Testin Antibody [NBP1-85072] - Staining of human cell line SiHa shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: Testin Antibody [NBP1-85072]

Western Blot: Testin Antibody [NBP1-85072]

Western Blot: Testin Antibody [NBP1-85072] - Western blot analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072] - Staining of human endometrium, liver, testis and urinary bladder using Anti-TES antibody NBP1-85072 (A) shows similar protein distribution across tissues to independent antibody NBP1-87987 (B).
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-85072] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Applications for Testin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Testin

Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. Multiple protein isoforms are encoded by transcript variants of this gene.

Alternate Names

DKFZP586B2022, MGC1146, TESS, TESS-2, TESTIN, testis derived transcript (3 LIM domains)

Gene Symbol

TES

Additional Testin Products

Product Documents for Testin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Testin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Testin Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Testin Antibody - BSA Free and earn rewards!

Have you used Testin Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies