Testin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87987

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Testin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%). Reactivity reported in scientific literature (PMID: 23227862)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Testin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Testin Antibody [NBP1-87987]

Immunocytochemistry/ Immunofluorescence: Testin Antibody [NBP1-87987]

Immunocytochemistry/Immunofluorescence: Testin Antibody [NBP1-87987] - Staining of human cell line SiHa shows localization to cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium, liver, testis and urinary bladder using Anti-TES antibody NBP1-87987 (A) shows similar protein distribution across tissues to independent antibody NBP1-85072 (B).
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987]

Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human liver shows no positivity in hepatocytes as expected.
Simple Western: Testin Antibody [NBP1-87987]

Simple Western: Testin Antibody [NBP1-87987]

Simple Western: Testin Antibody [NBP1-87987] - Simple Western lane view shows a specific band for TES in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Testin Antibody [NBP1-87987]

Simple Western: Testin Antibody [NBP1-87987]

Simple Western: Testin Antibody [NBP1-87987] - Electropherogram image(s) of corresponding Simple Western lane view. Testin antibody was used at 1:20 dilution on RT-4 lysate(s).
Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987]

Western Blot: Testin Antibody [NBP1-87987] - Analysis using Anti-TES antibody NBP1-87987 (A) shows similar pattern to independent antibody NBP1-85072 (B).
Testin Antibody - BSA Free Western Blot: Testin Antibody - BSA Free [NBP1-87987]

Western Blot: Testin Antibody - BSA Free [NBP1-87987]

Analysis in human cell lines A-431 and U-251MG using Anti-TES antibody. Corresponding TES RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for Testin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 57 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in IHC-P reported in scientific literature (PMID:34440214).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Testin

Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. Multiple protein isoforms are encoded by transcript variants of this gene.

Alternate Names

DKFZP586B2022, MGC1146, TESS, TESS-2, TESTIN, testis derived transcript (3 LIM domains)

Gene Symbol

TES

Additional Testin Products

Product Documents for Testin Antibody - BSA Free

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Product Specific Notices for Testin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Testin Antibody - BSA Free

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Protocols

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