Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TET2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 100 and 150 of human Tet Oncogene Family Member 2 using the numbering given in entry NP_001120680.1 (GeneID 54790).

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TET2 Antibody - BSA Free

Western Blot: TET2 Antibody [NBP2-32104]

Western Blot: TET2 Antibody [NBP2-32104]

Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TET2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-32104]

Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-32104]

Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-32104] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TET2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: TET2 Antibody [NBP2-32104]

Western Blot: TET2 Antibody [NBP2-32104]

Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TET2 antibody NBP2-32104 used for IP at 6 ug/mg lysate. TET2 was also immunoprecipitated by rabbit anti-TET2 antibody BL16766. For blotting immunoprecipitated TET2, NBP2-32104 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TET2 Antibody - BSA Free

Western Blot: TET2 Antibody - BSA Free [NBP2-32104] -

Proteomic analysis of m5C dioxygenases using 5-EC RNABPP.a 5-EC RNABPP workflow. Cells are metabolically labeled with 5-EC and crosslinked RNA-protein complexes are isolated by oligo(dT)-based enrichment. Enriched RNA-protein complexes are digested with RNase and then analyzed by mass spectrometry-based proteomics. b Volcano plot showing the enrichment of 5-EC reactive proteins. Experiment was performed independently in triplicate and protein abundance was quantified by spectral counting. Multiple student’s unpaired two-sided t-tests were used to assess statistical significance. c Western blot validation of ALKBH1, TET2, and MMP8. 293 Flp-In cells overexpressing the respective FLAG-tagged protein were treated with 1 mM 5-EC or DMSO and subjected to the RNABPP workflow before western blot analysis. Experiments were performed in triplicate with similar results. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35853884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TET2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections

Reviewed Applications

Read 1 review rated 4 using NBP2-32104 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TET2

Long Name

Methylcytosine dioxygenase TET2

Alternate Names

KIAA1546, Nbla00191

Gene Symbol

TET2

UniProt

NP_001120680.1 (Human)

Additional TET2 Products

Product Documents for TET2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TET2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TET2 Antibody - BSA Free

Customer Reviews for TET2 Antibody - BSA Free (1)

4 out of 5
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MOUSE FIBROBLAST AND KERATINOCYTE
    Species: Mouse
    Verified Customer | Posted 10/03/2014

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