TFCP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55972

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFCP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: QQQQQQKHEDGDSNGTFFVYHAIYL

Reactivity Notes

Mouse 84%, Rat 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFCP2 Antibody - BSA Free

Western Blot: TFCP2 Antibody [NBP2-55972]

Western Blot: TFCP2 Antibody [NBP2-55972]

Western Blot: TFCP2 Antibody [NBP2-55972] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: TFCP2 Antibody [NBP2-55972]

Immunocytochemistry/ Immunofluorescence: TFCP2 Antibody [NBP2-55972]

Immunocytochemistry/Immunofluorescence: TFCP2 Antibody [NBP2-55972] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
TFCP2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TFCP2 Antibody - BSA Free [NBP2-55972]

Chromatin Immunoprecipitation-exo-Seq: TFCP2 Antibody - BSA Free [NBP2-55972]

ChIP-Exo-Seq composite graph for Anti-TFCP2 (NBP2-55972) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TFCP2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFCP2

CP2c binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1. Functions as part of the SSP (stage selecto

Alternate Names

alpha-globin transcription factor CP2, CP2, LBP-1C, LSF1D, LSFLBP1C, SAA3 enhancer factor, SEF, TFCP2C, transcription factor CP2, Transcription factor LSF

Gene Symbol

TFCP2

Additional TFCP2 Products

Product Documents for TFCP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TFCP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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