TFII-I Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90010

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFII-I Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFII-I Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFII-I Antibody [NBP1-90010]

Immunocytochemistry/ Immunofluorescence: TFII-I Antibody [NBP1-90010]

Immunocytochemistry/Immunofluorescence: TFII-I Antibody [NBP1-90010] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010]

Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010]

Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010] - Staining of human stomach shows strong nuclear positivity in glandular cells.
TFII-I Antibody - BSA Free Western Blot: TFII-I Antibody - BSA Free [NBP1-90010]

Western Blot: TFII-I Antibody - BSA Free [NBP1-90010]

Analysis in human cell line SH-SY5Y.
TFII-I Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TFII-I Antibody - BSA Free [NBP1-90010]

Chromatin Immunoprecipitation-exo-Seq: TFII-I Antibody - BSA Free [NBP1-90010]

ChIP-Exo-Seq composite graph for Anti-GTF2I (NBP1-90010) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TFII-I Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-90010 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFII-I

TFII-I encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. The exon(s) encoding 5' UTR has not been fully defined, but this gene is known to contain at least 34 exons, and its alternative splicing generates 4 transcript variants.

Alternate Names

BAP-135,135kD, BAP135WBS, Bruton tyrosine kinase-associated protein 135, BTKAP1, DIWS, FLJ38776, FLJ56355, general transcription factor II, i, general transcription factor IIi, general transcription factor II-I, GTFII-I, IB291, SPINBTK-associated protein 135, TFII-ISRF-Phox1-interacting protein, WBSCR6, Williams-Beuren syndrome chromosome region 6

Gene Symbol

GTF2I

Additional TFII-I Products

Product Documents for TFII-I Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TFII-I Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TFII-I Antibody - BSA Free

Customer Reviews for TFII-I Antibody - BSA Free (1)

5 out of 5
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  • Name: Andres J Lopez-Contreras
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/03/2013
    TFII-I Antibody - BSA Free NBP1-90010

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