Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (Negative)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFIIIC Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2059 and 2109 of human general transcription factor IIIC, polypeptide 1 (general transcription factor IIIC, 220kDa subunit) using the numbering given in entry NP_001511.2 (GeneID 2

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23966877).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIIC Antibody - BSA Free

Western Blot: TFIIIC Antibody [NB100-60657]

Western Blot: TFIIIC Antibody [NB100-60657]

Western Blot: TFIIIC Antibody [NB100-60657] - Detection of Human GTF3C1/TFIIIC220 on HeLa whole cell lysate using NB100-60657. GTF3C1/TFIIIC220 was efficiently IPed by rabbit anti-GTF3C1/TFIIIC220 antibodies NB100-60655 and NB100-60656.
Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60657]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60657]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60657] - Human prostate carcinoma. Antibody used at a dilution of 1:1,000.
TFIIIC Antibody

Western Blot: Rabbit Polyclonal TFIIIC Antibody [NB100-60657] -

Western Blot: Rabbit Polyclonal TFIIIC Antibody [NB100-60657] - Western blot using GTF3C1 Antibody at concentration 1:1000. Image from a verified customer review.

Applications for TFIIIC Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 -1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:2000-1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31759822).

Reviewed Applications

Read 1 review rated 4 using NB100-60657 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFIIIC

General transcription factor 3C polypeptide 1 (GTF3C1/TFIIIC220) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. The functional contribution of GTF3C1/TFIIIC220 to the TFIIIC complex has not been fully determined.

Alternate Names

general transcription factor 3C polypeptide 1, general transcription factor IIIC, polypeptide 1 (alpha subunit), general transcription factor IIIC, polypeptide 1 (alpha subunit, 220kD ), general transcription factor IIIC, polypeptide 1, alpha 220kDa, TF3C-alpha, TFIIIC, TFIIIC 220 kDa subunit, TFIIIC box B-binding subunit, TFIIIC220DKFZp686A111, TFIIICalpha, Transcription factor IIIC 220 kDa subunit, Transcription factor IIIC subunit alpha

Entrez Gene IDs

2975 (Human)

Gene Symbol

GTF3C1

UniProt

NP_001511.2 (Human)

Additional TFIIIC Products

Product Documents for TFIIIC Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TFIIIC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TFIIIC Antibody - BSA Free

Customer Reviews for TFIIIC Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Good
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 01/30/2025
    Western blot using GTF3C1 Antibody at concentration 1:1000.
    I have used the antibody for western blot at concentration 1:1000.
    TFIIIC Antibody - BSA Free NB100-60657

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Protocols

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