TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34602

Clone DF1513 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DF1513

Format

Azide and BSA Free
View all TfR (Transferrin R) Primary Antibodies »

Product Specifications

Immunogen

KG1 acute myeloid leukemia cell line

Localization

Cell surface

Specificity

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32945).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

Western Blot: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Western Blot: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Western Blot: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).
Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]

Simple Western: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.

Applications for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Western Blot

0.25-0.5ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TfR (Transferrin R)

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free

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Protocols

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