TfR (Transferrin R) Antibody (DF1513)

Novus Biologicals | Catalog # NBP2-32945

Clone DF1513 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DF1513
View all TfR (Transferrin R) Primary Antibodies »

Product Specifications

Immunogen

KG1 acute myeloid leukemia cell line

Localization

Cell surface

Specificity

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).
Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.
TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] -

The effect of NO on iron handling gene expression in human placental villous explants. (A–E) qPCR results, (F–J) protein analyses. Representative blots are shown in (K). HO-1 and TfR mRNA levels are significantly increased in the group treated with NO compared to the untreated controls. Protein abundance of HO-1 is significantly increased in the group treated with NO compared to the untreated controls. No significant change in expression is seen in the rest of the genes studied. HO-1: heme oxygenase 1, HAMP: hepcidin antimicrobial peptide, TfR: transferrin receptor, DMT1: divalent metal transporter 1, FPN: ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, *** p < 0.001, ns = not significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] -

The effect of hypoxia on iron handling genes in response to NO in syncitialized BeWo cells (SCTs). (A–F) qPCR results and (G–K) protein analyses. Representative blots are shown in (K). Hypoxia alone did not significantly alter mRNA or protein levels of any of the studied iron homeostasis markers compared to normoxic controls. (A,B) The combination of hypoxia and NO resulted in a significant increase in HO-1 and HAMP mRNA levels, while NO increased HAMP mRNA levels significantly under hypoxic conditions. No significant effect of hypoxia or NO was seen in any of the other targets studied. HO-1: Heme Oxygenase 1, HAMP: Hepcidin antimicrobial peptide, TfR: Transferrin Receptor, TF: Transferrin, DMT1, Divalent metal transporter 1, FPN: Ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, * p < 0.05, ns = not significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] -

The effect of NO on iron handling genes in syncytialized BeWo cells. (A–E), qPCR results, and (E–J) protein analyses. Representative blots are shown in (K). HO-1 and HAMP mRNA levels are significantly increased in the group treated with NO compared to the untreated controls. Protein abundance of HO-1 is significantly increased in the group treated with NO compared to the untreated controls. No significant change in expression is seen in the rest of the genes studied. HO-1: heme oxygenase 1, HAMP: hepcidin antimicrobial peptide, TfR: transferrin receptor, DMT1: divalent metal transporter 1, FPN: ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, ** p < 0.01, **** p < 0.0001, ns = not significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TfR (Transferrin R) Antibody (DF1513)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat and K562 lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 133 kDa

Reviewed Applications

Read 1 review rated 4 using NBP2-32945 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TfR (Transferrin R)

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

UniProt

P02786 (Human)

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (DF1513)

Certificate of Analysis

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Product Specific Notices for TfR (Transferrin R) Antibody (DF1513)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TfR (Transferrin R) Antibody (DF1513)

Customer Reviews for TfR (Transferrin R) Antibody (DF1513) (1)

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  • TfR (Transferrin R) Antibody (DF1513)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreas tissue, Stomach tissue, Testis tissue and Skin tissue
    Species: Canine and Feline
    Verified Customer | Posted 06/29/2017
    Lane 1: Canine Pancreas Lane 2: Canine Skin Lane 3: Canine Stomach Lane 4: Canine Testis Lane 5: Feline Pancreas Lane 6: Feline Skin Lane 7: Feline Stomach Lane 8: Feline Testis
    This antibody will be used for IHC-P in both canine and feline tissue (results pending)
    TfR (Transferrin R) Antibody (DF1513) NBP2-32945

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