TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80976

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OX26

Format

Azide and BSA Free
View all TfR (Transferrin R) Primary Antibodies »

Product Specifications

Immunogen

PHA activated rat lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free

Western Blot: TfR (Transferrin R) Antibody (OX26)Azide and BSA Free [NBP2-80976]

Western Blot: TfR (Transferrin R) Antibody (OX26)Azide and BSA Free [NBP2-80976]

Western Blot: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenchi
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen lymphocytes with mouse anti rat CD71. Image from the standard format of this antibody.
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976]

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free [NBP2-80976] - Staining of rat spleen cells with mouse anti rat CD71: RPE. Image from the standard format of this antibody.

Applications for TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TfR (Transferrin R)

OX26 TfR clone is useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. In comes in a variety of pre-conjugated fluorophores for histochemical and flow applications.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TfR (Transferrin R) Antibody (OX26) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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