TGF-beta RII Antibody (DMC467) - Chimeric

Novus Biologicals | Catalog # NBP3-48350

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC467
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Product Specifications

Immunogen

TGF-beta RII recombinant protein expressed in mammalian cells (Accession# P37173). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for TGF-beta RII Antibody (DMC467) - Chimeric

TGF-beta RII Antibody (DMC467) - Chimeric

Flow Cytometry: TGF-beta RII Antibody (DMC467) - Chimeric [NBP3-48350]

Flow cytometry analysis with Anti-TGF-beta RII (DMC467) on HEK293 cells transfected with human TGF-beta RII (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
TGF-beta RII Antibody (DMC467) - Chimeric

Flow Cytometry: TGF-beta RII Antibody (DMC467) - Chimeric [NBP3-48350]

Flow cytometry analysis of antigen binding of anti-human TGFBR2 mAb(NBP3-48350). (A) NBP3-48350 does not bind to CHO-S cells that do not express TGFBR2. (B) A clear peak shift of NBP3-48350 was seen compared to the control when incubated with TGFBR2-expressing hepG2 cells, indicating strong binding of NBP3-48350 to TGFBR2. Antibodies were incubated at 5 ug/ml.

Applications for TGF-beta RII Antibody (DMC467) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

TGF beta Receptor II is a total of three members of the TGFbeta family, TGFbeta1, TGFbeta2 and TGFbeta3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbetas mediate their activity by high affinity binding to the type II receptor (TGFbeta RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses the type II receptor requires both its kinase activity and association with a TGFbeta-binding protein, designated the type I receptor. Two independent groups have recently described the cloning and sequence analysis of genes encoding TGFbeta type I receptor proteins designated ALK-5 (TbetaR-1) and TSR-1, respectively.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Gene Symbol

TGFBR2

Additional TGF-beta RII Products

Product Documents for TGF-beta RII Antibody (DMC467) - Chimeric

Certificate of Analysis

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Product Specific Notices for TGF-beta RII Antibody (DMC467) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TGF-beta RII Antibody (DMC467) - Chimeric

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  • Q:

    Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

    A:

    Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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