Recombinant Human TGF-beta RII Protein

R&D Systems | Catalog # 241-R2

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human TGF-beta RII Protein (241-R2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P37173.2

Applications

Bioactivity
View all TGF-beta RII Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TGF-beta RII protein
Ile24-Asp159

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile24

Predicted Molecular Mass

15 kDa

SDS-PAGE

Multiple bands between 20-35 kDa, reducing conditions

Activity

Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 1.00-10.0 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.

Reviewed Applications

Read 3 reviews rated 4.7 using 241-R2 in the following applications:

Scientific Data Images for Recombinant Human TGF-beta RII Protein

Graph showing dose-dependent bioactivity of human TGF-beta RII protein

Bioactivity of Human TGF-beta RII

Recombinant human TGF-beta RII (241-R2) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1.00-10.0 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 241-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 241-R2/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70-85 kDa), and type III (250-350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References

  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

P37173.2

Additional TGF-beta RII Products

Product Documents for Recombinant Human TGF-beta RII Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 241-R2 (with carrier)
Download SDS 241-R2/CF (carrier free)

Product Specific Notices for Recombinant Human TGF-beta RII Protein

For research use only

Citations for Recombinant Human TGF-beta RII Protein

Customer Reviews for Recombinant Human TGF-beta RII Protein (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Recombinant Human TGF-beta RII Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/27/2020
  • Recombinant Human TGF-beta RII Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/27/2020
  • Recombinant Human TGF-beta RII Protein
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    Application: Cell migration/motility
    Verified Customer | Posted 10/05/2020

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FAQs for Recombinant Human TGF-beta RII Protein

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  • Q:

    Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

    A:

    Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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