Recombinant Human TGF-beta RII Protein

Carrier Free

Catalog # Availability Size / Price Qty
241-R2-025/CF

With Carrier

Catalog # Availability Size / Price Qty
241-R2-025
Graph showing dose-dependent bioactivity of human TGF-beta RII protein
1 Image
Product Details
Citations (4)
FAQs
Reviews (3)

Recombinant Human TGF-beta RII Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.
Source
Mouse myeloma cell line, NS0-derived human TGF-beta RII protein
Ile24-Asp159
Accession #
N-terminal Sequence
Analysis
Ile24
Predicted Molecular Mass
15 kDa
SDS-PAGE
Multiple bands between 20-35 kDa, reducing conditions

Product Datasheets

241-R2 (with carrier)

241-R2/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

241-R2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

241-R2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Human TGF-beta  RII View Larger

Recombinant human TGF-beta RII (241-R2) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70-85 kDa), and type III (250-350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References
  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Citations for Recombinant Human TGF-beta RII Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation.
    Authors: Graham J, Chamberland A, Lin Q, Li X, Dai D, Zeng W, Ryan M, Rivera-Bermudez M, Flannery C, Yang Z
    PLoS ONE, 2013;8(9):e74094.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism.
    Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM
    Cancer Res., 2008;68(2):561-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
    Authors: Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE
    J. Cell. Physiol., 2006;207(1):40-8.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
    Authors: Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
    91, 2002;1590(1):1-15.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

    • Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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Reviews for Recombinant Human TGF-beta RII Protein

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Human TGF-beta RII Protein
By Anonymous on 10/27/2020
Application: Enzymatic activity in vitro

Recombinant Human TGF-beta RII Protein
By Anonymous on 10/27/2020
Application: Binding assay/Protein-protein interaction

Recombinant Human TGF-beta RII Protein
By Anonymous on 10/05/2020
Application: Cell migration/motility