TGF-beta 1, 2, 3 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1835P
Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (2)
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TGF-beta 1, 2, 3 PE-conjugated Antibody Summary

Specificity
Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
Source
Monoclonal Mouse IgG1 Clone # 1D11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Bovine bone-derived TGF-beta 1 and TGF-beta 2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. View Larger

Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1, 2, 3 PE‑conjugated Mono­clonal Antibody (Catalog # IC1835P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References
  1. Ayala A. et al. (1992) FASEB J. 6:A1604.
  2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
  3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
Long Name
Transforming Growth Factor beta 1, 2, and 3
Alternate Names
CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Product Datasheets

Citations for TGF-beta 1, 2, 3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction.
    Authors: Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z
    Eur J Immunol, 2013;43(9):2461-72.
    Species: Mouse
    Sample Types: Exosomes
    Applications: Flow Cytometry
  2. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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