TGF‑ beta 1, 2, 3 PE‑conjugated Antibody

  • Specificity
    Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 1D11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Bovine bone-derived TGF-beta 1 and TGF-beta 2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TGF-beta 1, 2, 3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 PE-conjugated Mono­clonal Antibody (Catalog # IC1835P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1, 2, 3
TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  • References:
    1. Ayala A. et al. (1992) FASEB J. 6:A1604.
    2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
    3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
  • Long Name:
    Transforming Growth Factor beta 1, 2, and 3
  • Alternate Names:
    CED; DPD1; LAP; TGFB; TGFB1; TGFbeta; transforming growth factor, beta 1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction.
    Authors: Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z
    Eur J Immunol, 2013;43(9):2461-72.
    Species: Mouse
    Sample Type: exosomes
    Application: Flow Cytometry,
  2. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Cytometry,


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