Thrombospondin-1 Antibody (A6.1)

Novus Biologicals | Catalog # NB100-2059

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize, IF/IHC, PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A6.1
View all Thrombospondin-1 Primary Antibodies »

Product Specifications

Immunogen

BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Localization

Secretory granules, Golgi complex, endoplasmic reticulum

Specificity

This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Thrombospondin-1 Antibody (A6.1)

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpg
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0013.jpg
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0014.jpg
Thrombospondin-1 Antibody (A6.1)

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] -

Thrombospondin-1, CD36, and CD61 protein expression is down-regulated in active celiac disease.Representative photomicrographs showing Thrombospondin-1 (A), CD36 (B) and CD61 (C) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean ± SD of all experiments.
Thrombospondin-1 Antibody (A6.1)

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] -

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Reduced levels of vascular & pericyte markers in MMP12KO mice.A. IHC staining of vWF, TSP-1 & PDGFR beta was performed on paraffin sections from the heart of PBS & Ang II treated WT & MMP12KO mice. B. IHC staining of vWF, & PDGFR beta was performed on paraffin sections from the skin of PBS & Ang II treated WT & MMP12KO mice. Representative photographs are shown from three animals per group. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Thrombospondin-1 Antibody (A6.1)

Application
Recommended Usage

Immunohistochemistry

1:25-1:50

Immunohistochemistry-Paraffin

1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THBS1

UniProt

P07996

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody (A6.1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thrombospondin-1 Antibody (A6.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Thrombospondin-1 Antibody (A6.1)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Thrombospondin-1 Antibody (A6.1)

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  • Q: Could you please tell me the buffer formulation and concentration of sodium azide for this antibody? 

    A: Yes, Sodium Azide is less than 0.1%, and composition of buffer is PBS. 

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