Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25720

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1194 expressed in HEK293

Format

Azide and BSA Free
View all Thrombospondin-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombospondin-2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -

Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Thrombospondin 2 antibody [HL1194] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:500. Blue: Fluoroshield with DAPI.
Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -

ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.
Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -

Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -

ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.
Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -

Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombospondin-2

Thrombospondin 2 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It can bind to fibrinogen, fibronectin, laminin and type V collagen. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.

Alternate Names

THBS2, Thrombospondin2, TSP-2

Gene Symbol

THBS2

Additional Thrombospondin-2 Products

Product Documents for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail